The Arabidopsis TRM1-TON1 Interaction Reveals a Recruitment Network Common to Plant Cortical Microtubule Arrays and Eukaryotic Centrosomes
The Arabidopsis TRM1-TON1 Interaction Reveals a Recruitment Network Common to Plant Cortical Microtubule Arrays and Eukaryotic Centrosomes
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DOI：
10.1105/tpc.111.089748
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发表时间：
2012-01-01
期刊：
影响因子：
11.6
通讯作者：
Pastuglia, Martine
中科院分区：
文献类型：
--
作者：
Drevensek, Stephanie;Goussot, Magali;Pastuglia, Martine
陆地植物细胞组装微管阵列没有一个明显的微管组织中心，如中心体。在拟南芥中，TONNEAU 1（TON 1）蛋白与FOP（一种人类中心体蛋白）具有相似性，对皮层微管组织至关重要。我们已经确定了一个新的超家族的34个蛋白保守的陆地植物，TON 1招募基序（TRM）蛋白，其中共享6个短的保守基序，包括TON 1相互作用的基序存在于所有的TRM。该家族的原型成员TRM 1是一种微管相关蛋白，其定位于皮质微管并在体外结合微管。并非所有的TRM蛋白都能结合微管，这表明该家族的功能多样性。此外，我们表明，TRM 1在体内与TON 1相互作用，并能够通过其C-末端TON 1相互作用基序将TON 1靶向皮质微管。有趣的是，在与FOP相互作用的人类中心体蛋白CAP 350中发现了三个TRM基序，并且CAP 350的C-末端M2基序负责中心体处的FOP募集。此外，我们发现TON 1可以在酵母中与人CAP 350 M2基序相互作用。两者合计，我们的研究结果表明，在植物细胞中的真核中心体组件的保护。
Land plant cells assemble microtubule arrays without a conspicuous microtubule organizing center like a centrosome. In Arabidopsis thaliana, the TONNEAU1 (TON1) proteins, which share similarity with FOP, a human centrosomal protein, are essential for microtubule organization at the cortex. We have identified a novel superfamily of 34 proteins conserved in land plants, the TON1 Recruiting Motif (TRM) proteins, which share six short conserved motifs, including a TON1-interacting motif present in all TRMs. An archetypal member of this family, TRM1, is a microtubule-associated protein that localizes to cortical microtubules and binds microtubules in vitro. Not all TRM proteins can bind microtubules, suggesting a diversity of functions for this family. In addition, we show that TRM1 interacts in vivo with TON1 and is able to target TON1 to cortical microtubules via its C-terminal TON1 interaction motif. Interestingly, three motifs of TRMs are found in CAP350, a human centrosomal protein interacting with FOP, and the C-terminal M2 motif of CAP350 is responsible for FOP recruitment at the centrosome. Moreover, we found that TON1 can interact with the human CAP350 M2 motif in yeast. Taken together, our results suggest conservation of eukaryotic centrosomal components in plant cells.