Effect of Flos carthami on stress-activated protein kinase activity in the isolated reperfused rat heart
Effect of Flos carthami on stress-activated protein kinase activity in the isolated reperfused rat heart
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DOI：
10.1023/a:1017266628572
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发表时间：
2000-04
影响因子：
4.3
通讯作者：
Y. Siow;P. Choy;W. Leung;O. Karmin
中科院分区：
文献类型：
--
作者：
Y. Siow;P. Choy;W. Leung;O. Karmin
缺血/再灌注引起的心肌细胞凋亡性死亡是心脏疾病的主要并发症之一。已有研究表明，缺血/再灌注可导致应激激活蛋白(SAP)激酶和p38/再激活激酶(p38/RK)的激活。在这项研究中，我们研究了红花提取物(FC)对SAP激酶的直接影响。在诱导大鼠全心缺血和随后的再灌流前，用含FC提取物的培养液以朗宁多夫模式灌流大鼠离体心，SAP激酶活性被抑制95%。未经处理的缺血/再灌流心脏显示SAP激酶活性升高57%。此外，还检测了这些FC提取物对SAP激酶的体外作用。当浓度为10μg/ml时，Fc水提物对缺血心肌组织中SAP激酶活性的抑制率为50%。我们的结果表明，Fc既影响了SAP激酶与c-jun的相互作用，也影响了磷酸转移酶反应。这些结果清楚地表明，红花提取物对SAP激酶有抑制作用。给予FC提取物可能导致对缺血/再灌流诱导的SAP激酶激活的凋亡效应的调节。
The apoptotic death of cardiomyocytes due to ischemia/reperfusion is one of the major complications of heart disease. Ischemia/reperfusion has been shown to lead to the activation of the stress-activated protein (SAP) kinases and the p38/reactivating kinase (p38/RK). In this study, the direct effect of an aqueousFlos carthami(FC) extract on SAP kinases was investigated. When isolated rat hearts were perfused by Langendorff mode with media containing FC extract prior to the induction of global ischemia and the subsequent reperfusion, SAP kinase activity was inhibited 95%. Untreated ischemic/reperfused hearts showed a 57% elevation in the activity of SAP kinase. Thein vitroeffect of these FC extracts on SAP kinase was also tested. At a concentration of 10 μg/ml, the aqueous FC extract resulted in 50% inhibition of SAP kinase activity in ischemic heart tissue. Our results showed that FC affected both the interaction of SAP kinase with c-jun as well as the phosphotransferase reaction. These results clearly demonstrate that extracts fromFlos carthamiexerted inhibitory effects on SAP kinase. The administration of the FC extract may lead to a modulation of the apoptotic effect of SAP kinase activation induced during ischemia/reperfusion.