Function Identification of Bovine ACSF3 Gene and Its Association With Lipid Metabolism Traits in Beef Cattle.
Function Identification of Bovine ACSF3 Gene and Its Association With Lipid Metabolism Traits in Beef Cattle.
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DOI：
10.3389/fvets.2021.766765
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发表时间：
2021
影响因子：
3.2
通讯作者：
Yang R
中科院分区：
文献类型：
--
作者：
He W;Fang X;Lu X;Liu Y;Li G;Zhao Z;Li J;Yang R
酰基辅酶a合成酶家族成员3 （ACSF3）进行线粒体脂肪酸合成II的第一步，即丙二酸酯和甲基丙二酸酯（在较小程度上）与辅酶a的连锁。丙二酰辅酶A （malonyl-CoA）是哺乳动物脂肪酸生物化学的中心代谢物，在细胞质中产生和利用。在本研究中，我们通过沉默和过表达ACSF3，在细胞水平上验证了ACSF3的表达与甘油三酯（TGs）的产生之间的关系。随后，通过Sanger测序，在牛ACSF3功能域发现了5个多态性，并通过方差分析和多重比较的方法分析了ACSF3多态性与中国西门门牛经济性状和脂肪酸组成的关系。结果表明，ACSF3的表达促进了牛乳腺上皮细胞和牛胎儿成纤维细胞中甘油三酯的合成。进一步的关联分析也表明AG基因型（g.14211090）的个体g> A)与肌内脂肪脂肪酸组成（亚油酸、α-亚麻酸、花生四烯酸含量较高）显著相关，CTCAG单倍型个体与肌内脂肪脂肪酸组成（亚油酸含量较高）显著相关。g.14211055 AA基因型个体A b> G和G .14211090在中国西门塔尔牛种群中，G > A与更大的眼肌面积密切相关。ACSF3在细胞内甘油三酯和长链多不饱和脂肪酸水平的调控中发挥着关键作用，其多态性可作为未来肉牛肌内脂肪沉积性状的标记辅助选择育种的有用分子标记。
Acyl-CoA synthetase family member 3 (ACSF3) carries out the first step of mitochondrial fatty acid synthesis II, which is the linkage of malonate and, to a lesser extent, methylmalonate onto CoA. Malonyl-coenzyme A (malonyl-CoA) is a central metabolite in mammalian fatty acid biochemistry that is generated and utilized in the cytoplasm. In this research, we verified the relationship between expression of the ACSF3 and the production of triglycerides (TGs) at the cellular level by silencing and over-expressing ACSF3. Subsequently, through Sanger sequencing, five polymorphisms were found in the functional domain of the bovine ACSF3, and the relationship between ACSF3 polymorphism and the economic traits and fatty acid composition of Chinese Simmental cattle was analyzed by a means of variance analysis and multiple comparison. The results illustrated that the expression of ACSF3 promoted triglyceride synthesis in bovine mammary epithelial cells and bovine fetal fibroblast cells. Further association analysis also indicated that individuals with the AG genotype (g.14211090 G > A) of ACSF3 were significantly associated with the fatty acid composition of intramuscular fat (higher content of linoleic acid, α-linolenic acid, and arachidonic acid), and that CTCAG haplotype individuals were significantly related to the fatty acid composition of intramuscular fat (higher linoleic acid content). Individuals with the AA genotypes of g.14211055 A > G and g.14211090 G > A were substantially associated with a larger eye muscle area in the Chinese Simmental cattle population. ACSF3 played a pivotal role in the regulation of cellular triacylglycerol and long-chain polyunsaturated fatty acid levels, and polymorphism could serve as a useful molecular marker for future marker-assisted selection in the breeding of intramuscular fat deposition traits in beef cattle.
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影响因子：
3.3
作者：
Bernal Rubio, Y. L.;Gualdron Duarte, J. L.;Steibel, J. P.
通讯作者：
Steibel, J. P.
影响因子：
4.8
作者：
Witkowski, Andrzej;Thweatt, Jennifer;Smith, Stuart
通讯作者：
Smith, Stuart
影响因子：
4.1
作者：
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通讯作者：
Kastaniotis, Alexander J.
影响因子：
7.2
作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
Eisenbeis, SA