Fed-batch fermentation with intermittent gas stripping using immobilized Clostridium acetobutylicum for biobutanol production from corn stover bagasse hydrolysate
Fed-batch fermentation with intermittent gas stripping using immobilized Clostridium acetobutylicum for biobutanol production from corn stover bagasse hydrolysate
复制标题
使用固定化丙酮丁醇梭菌进行间歇式气提分批补料发酵，从玉米秸秆甘蔗渣水解产物中生产生物丁醇
DOI：
10.1016/j.bej.2017.05.006
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发表时间：
2017-09-15
影响因子：
3.9
通讯作者：
Qin, Peiyong
中科院分区：
文献类型：
--
作者：
Cai, Di;Chen, Changjing;Qin, Peiyong
以玉米秸秆蔗渣水解液为底物进行丙酮-丁醇-乙醇（ABE）补料分批发酵，并采用间歇气提法原位脱除产物。以丙酮丁醇梭菌（Clostridium acetobutylicum ABE-P 1201）为出发菌株，对ABE进行了诱变。结果，由97.6g/L的总可发酵糖（75.3g/L的葡萄糖和22.3g/L的木糖）产生18.6g/L的最终累积丁醇浓度（总ABE：28.3g/L），ABE产率为83.2g/kg CSB。在整合方法中获得的气体汽提冷凝物含有77-136 g/L的丁醇和115-220 g/L的总ABE。(C)2017爱思唯尔B. V.保留所有权利。
Corn stover bagasse hydrolysate was used as the substrate for fed-batch acetone-butanol-ethanol (ABE) fermentation and in situ product removal by intermittent gas stripping. The mutant strain Clostridium acetobutylicum ABE-P 1201 was inoculated for ABE production. As results, a final cumulative butanol concentration of 18.6 g/L (total ABE: 28.3 g/L) was produced from 97.6 g/L of total fermentable sugars (75.3 g/L of glucose and 22.3 g/L of xylose), with an ABE yield of 83.2 g/kg of CSB. The gas stripping condensate obtained in the integrated process contained 77-136 g/L of butanol and 115-220 g/L of total ABE. (C) 2017 Elsevier B.V. All rights reserved.