A regular fucan sulfate from Stichopus herrmanni and its peroxide depolymerization: Structure and anticoagulant activity
A regular fucan sulfate from Stichopus herrmanni and its peroxide depolymerization: Structure and anticoagulant activity
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赫曼海参中的常规岩藻聚糖硫酸盐及其过氧化物解聚：结构和抗凝血活性
DOI：
10.1016/j.carbpol.2020.117513
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发表时间：
2021-03-15
影响因子：
11.2
通讯作者：
Zhao, Jinhua
中科院分区：
文献类型：
--
作者：
Li, Xiaomei;Li, Shanni;Zhao, Jinhua
海洋硫酸多糖因其结构和生物活性的多样性而引起人们的广泛关注。过氧化物解聚是多糖结构分析和构效关系研究中常用的方法。然而，确定解聚过程和降解产物的确切结构仍然是一个相当大的挑战。报道了海参中岩藻聚糖硫酸酯（FS）及其过氧化物降解产物（dFS）的结构。FS具有高度规则的结构，{-3）-L-Fuc（2S）-（α 1-}n。从dFS中纯化的寡糖的结构分析表明，过氧化物降解涉及糖苷键的断裂和糖残基的还原端的氧化修饰，而没有观察到糖环的断裂。FS和系列dFS均表现出显著的抗凝活性，这是由于它们在肝素辅因子II存在下的抗凝血酶作用，并且它们的效力与它们的分子大小有关，类似于20 kDa的dFS表现出最强的活性。
Marine sulfated polysaccharides have aroused widespread concern for their various structures and bioactivities. Peroxide depolymerization is a common strategy in analysis of structures and structure-activity relationships of polysaccharides. However, confirming the depolymerization process and exact structures of the degradation products is still a considerable challenge. This study reported the structures of a fucan sulfate (FS) from sea cucumber Stichopus herrmanni and its depolymerized products (dFS) prepared by peroxide degradation. The FS was elucidated with a highly regular structure, {-3)-L-Fuc(2S)-(alpha 1-}n. Structure analysis of oligosaccharides purified from dFS suggested that peroxide degradation involved in cleavage of glycosidic bonds and oxidative modification of reducing end of sugar residue, while no break in sugar ring was observed. Both FS and series of dFSs exhibited significant anticoagulant activities due to their anti-thrombin effects in presence of heparin cofactor II and their potencies were related to their molecular sizes, dFS with similar to 20 kDa showed the strongest activity.