Single-cell MALDI: a new tool for direct peptide profiling
Single-cell MALDI: a new tool for direct peptide profiling
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DOI：
10.1016/s0167-7799(00)01427-x
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发表时间：
2000-04-01
影响因子：
17.3
通讯作者：
Sweedler, JV
中科院分区：
文献类型：
--
作者：
Li, LJ;Garden, RW;Sweedler, JV
基质辅助激光解吸电离（MALDI）质谱（MS）是一种快速灵敏的分析方法，非常适合从复杂样品中获得肽和蛋白质的分子量。MALDHMS可以从单细胞和小组织样品中分析肽和蛋白质，而不需要大量的样品制备，除了细胞分离和基质应用。提出了肽识别和翻译后修饰表征的策略。此外，MALDHMS技术，包括原位肽测序以及直接的空间映射的肽在细胞和组织中的几个最近的增强进行了讨论。
Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDHMS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDHMS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed.