Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS
Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS
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DOI：
10.1016/j.jsbmb.2010.03.030
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发表时间：
2010-08-01
影响因子：
4.1
通讯作者：
Matsuzaki, Yasushi
中科院分区：
文献类型：
--
作者：
Honda, Akira;Miyazaki, Teruo;Matsuzaki, Yasushi
高效液相色谱-串联质谱 (HPLC-MS/MS) 是对生物样品中的化合物进行微量分析的强大方法。与气相色谱-质谱（GC-MS）相比，该方法更广泛地适用于各种化合物，并且通常不需要在分析前进行衍生化步骤。然而，在分析中性甾醇时，由于甾醇相对不易电离，因此常规 HPLC-MS/MS 方法的灵敏度并不优于 GC-MS。在这篇综述中，我们介绍了用于非胆固醇甾醇定量的 HPLC-MS/MS 分析的最新进展。通过在传统程序中添加有效的衍生化步骤，HPLC-MS/MS 进行的甾醇分析的灵敏度超过了 GC-MS 获得的分析结果。此外，通过选择反应监测（SRM）和每种甾醇的彻底色谱分离，该方法具有足够的特异性。 (C) 2010 Elsevier Ltd. 保留所有权利。
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a powerful method for the microanalysis of compounds in biological samples. Compared with gas chromatography-mass spectrometry (GC-MS), this method is more broadly applicable to various compounds and usually does not require a derivatization step before analysis. However, when neutral sterols are analyzed, the sensitivities of usual HPLC-MS/MS method are not superior to those of GC-MS because the sterols are relatively resistant to ionization. In this review, we introduce the recent development of HPLC-MS/MS analysis for the quantification of non-cholesterol sterols. By adding an effective derivatization step to the conventional procedure, sterol analysis by HPLC-MS/MS surpassed that obtained by GC-MS in sensitivity. In addition, sufficient specificity of this method was achieved by selected reaction monitoring (SRM) and thorough chromatographic separation of each sterol. (C) 2010 Elsevier Ltd. All rights reserved.