Exposure to sublethal blast overpressure reduces the food intake and exercise performance of rats
Exposure to sublethal blast overpressure reduces the food intake and exercise performance of rats
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DOI：
10.1016/s0300-483x(97)03656-1
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发表时间：
1997-07-25
期刊：
影响因子：
4.5
通讯作者：
Widholm, J
中科院分区：
文献类型：
--
作者：
Bauman, RA;Elsayed, N;Widholm, J
暴露在爆炸超压下通常会对主要器官系统造成全身损伤，尤其是肺和胃肠道等含气体的器官。本研究的目的是利用大鼠的食物摄入和滚轮运动作为亚致死爆炸超压对这些器官系统造成的可能更微妙损害的行为相关性。为此，所有大鼠暴露于12小时的光/暗循环中，仅在黑暗时期提供食物。在暴露之前，(E)运动组的大鼠被要求对食物颗粒进行五次活动轮旋转；在大鼠进食以外的时间发生的车轮转动被单独记录下来，并标记为运动跑步。在(S)静坐组和(A)麻醉组，不可能进行轮跑，并且要求大鼠对单个颗粒执行五次杠杆按压。使用压缩空气驱动的激波管将大鼠暴露在超大气压力波中。管被聚酯膜分成两部分，膜的厚度决定了超压的峰值和持续时间。所有大鼠均以50mg /kg苯巴比妥麻醉。在达到深度麻醉平面后，他们分别被绑在一个袜子里，穿过休克管的一端。在初步试验中，膜厚度为1000（埃）μ m， L组（死亡）大鼠暴露于129 kPa（峰值振幅）的超压波下。6只老鼠中有3只在这种压力下存活了下来；所有非幸存者的肺部和胃肠道病理都很明显。E组和S组大鼠分别用500埃的膜测得83 kPa的峰值。所有的老鼠暴露在这个较低的峰值压力下都存活了下来。爆炸当天，E组暗期前3 h采食量的相对减少量显著大于S组和A组；E组和S组的摄取量继续减少4天。体重没有明显的影响。在暴露当天和随后的恢复期间，轮毂运动也显著减少。这些初步研究结果表明，暴露在亚致死爆炸超压下可能会减少食物消耗和运动表现，这可能是胃肠道和肺部受损的结果。(C) 1997爱思唯尔科学爱尔兰有限公司
Exposure to blast overpressure can typically inflict generalized damage on major organ systems, especially gas-containing organs such as the lungs and the gastrointestinal tract. The purpose of the present study was to use rat's food intake and exercise wheel running as behavioral correlates of the perhaps more subtle damage to these organ systems induced by sublethal blast overpressure. Toward this end, all rats were exposed to a 12-h light/dark cycle and food was available only in the dark period. Prior to exposure, rats in the (E)xercise group were required to execute five rotations of an activity wheel for a food pellet; wheel turns that occurred at times other than when a rat was feeding were recorded separately and labeled exercise running. In the (S)edentary and (A)nesthesia groups, wheel running was not possible and rats were required to execute five leverpresses for a single pellet. A compressed air-driven shock tube was used to expose rats to a supra-atmospheric wave of air pressure. The tube was separated into two sections by a polyester membrane, the thickness of which determined peak and duration of overpressure. All rats were anesthetized with 50 mg/kg of phenobarbital. After reaching a deep plane of anesthesia, they were individually tied in a stockinet across one end of the shock tube. In preliminary tests, the membrane thickness was 1000 (Angstrom)ngstroms and rats in Group L(ethality) were exposed to a 129 kPa (peak amplitude) wave of overpressure. Three of six rats survived exposure to this peak pressure; pathology was evident in the lungs and gastrointestinal tract of all non-survivors. Rats in Groups E and S were tested with a 500 Angstrom membrane, which resulted in an 83 kPa peak amplitude. All rats survived exposure to this lower peak pressure. On the day of exposure to blast, the relative reduction of intake during the first 3 h of the dark period was significantly greater for Group E than for Groups S and A; the intake of Groups E and S remained reduced for four additional recovery days. Bodyweight was not significantly affected. Exercise wheel running also was reduced significantly on the day of exposure and during subsequent recovery days. These preliminary findings suggest that exposure to sublethal blast overpressure can reduce food consumption and exercise performance, perhaps as a consequence of damage to the gastrointestinal tract and lungs. (C) 1997 Elsevier Science Ireland Ltd.