Process Intensification and Characterization of Agarose Gel Activation with Epichlorohydrin
Process Intensification and Characterization of Agarose Gel Activation with Epichlorohydrin
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发表时间：
2007
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通讯作者：
Sun Yan
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作者：
Sun Yan
琼脂糖凝胶的环氧密度通常受到环氧氯丙烷（ECH）的低水溶性及其在活化过程中水解的限制。在这项工作中，引入了一种亲水性有机试剂二甲亚砜（DMSO）来增强琼脂糖凝胶的环氧活化。结果表明，DMSO溶液中环氧氯丙烷和琼脂糖凝胶悬浮液的相界面消失。因此，ECH 在 DMSO 中的高溶解度增强了环氧树脂的活化。在 10%(-) 环氧氯丙烷和 0.8 mol/L 氢氧化钠条件下，最佳环氧密度超过 100 μmol/mL。活化的凝胶没有表现出任何性能损失。配体5-氨基吲哚在凝胶上的偶联密度达到95.3 μmol/mL，比普通环氧活化琼脂糖凝胶的偶联密度高75%。它在制备具有高配体密度的功能性琼脂糖凝胶方面表现出令人着迷的潜力。
The epoxy density of agarose gels is generally limited by low water-solubility of epichlorohydrin (ECH) and its hydrolysis during the activation process. In this work, a hydrophilic organic agent, dimethyl sulfoxide (DMSO), was introduced to enhance the epoxy activation of agarose gel. The results showed that the phase interface of epichlorohydrin and agarose gel suspension was diminished in the DMSO solution. Thus the epoxy activation was enhanced by a high ECH solubility in DMSO. An optimal epoxy density over 100 μmol/mL was achieved at 10%(-) epichlorohydrin and 0.8 mol/L sodium hydroxide. The activated gel did not show any loss in performance. The coupling density of a ligand, 5-aminoindole, on the gel reached 95.3 μmol/mL, being 75% higher than that obtained with common epoxy-activated agarose gel. It exhibited intriguing potential in the preparation of functional agarose gel with a high density of ligand.