[Demonstration of antibodies against HBs antigen with the modified MICROELISA-hepanostika-test (author's transl)].
[Demonstration of antibodies against HBs antigen with the modified MICROELISA-hepanostika-test (author's transl)].
复制标题
[用改良的 MICROELISA-hepanostika-test 演示抗 HBs 抗原的抗体（作者的翻译）]。
DOI：
10.1055/s-0028-1129360
复制
发表时间：
1978
影响因子：
0.6
通讯作者：
H. Köhler
中科院分区：
文献类型：
--
作者：
W. Lange;H. Köhler
在Hepanostika试验的各种改进中，一种简单、灵敏和可重复的试验被描述为是最好的。在689份血液透析患者血清中，Hepanostika试验中有110份抗HBs抗原抗体，Ausab试验中有150份抗HBs抗原抗体。在Hepanostika试验中未显示的那些抗体具有相对低的浓度（每分钟平均脉冲4.3 X NCx）。在Hepanostika和Ausab试验中均为阳性的血清含有较高浓度的抗体（平均脉冲/min 29.5 X NCx）。在689份血清中，只有11份在迁移电泳试验中呈阳性。改良Hepanostika试验的一个特别优点是，可以在同一平板上同时显示HBs抗原和抗HBs抗原的抗体。用此方法检测了200份血清。在HBS-II-125检测中检测相同血清的HBs抗原，并在Ausab检测中检测抗HBs抗原的抗体。Hepanostika试验阳性5例，奥地利试验阳性4例。根据Hepanostika试验，43份血清含有抗HBs抗原的抗体，而根据Ausab试验，45份血清含有抗HBs抗原的抗体。仅在Ausab试验中呈阳性的两个仅为弱阳性。
Among various modifications of the Hepanostika test a simple, sensitive and reproducible one is described as being the best. Of 689 sera from patients in a haemodialysis unit 110 had antibodies against HBs antigen in the Hepanostika test and 150 in the Ausab test. Those antibodies which were not demonstrated in the Hepanostika test had a relatively low concentration (mean impulses per minutes 4.3 X NCx). Those sera which were positive in both the Hepanostika and Ausab test contained antibodies in higher concentrations (mean impulse/min 29.5 X NCx). Only 11 of the 689 sera were positive in the migration-electrophoresis test. A particularly advantage of the modified Hepanostika test is that both HBs antigen and antibodies against HBs antigen can be demonstrated simultaneously on the same plate. Using this method 200 sera were examined. The same sera were tested in the Austria-II-125 test for HBs antigen and the Ausab test for antibodies against HBs antigen. The Hepanostika test was positive in five, the Austria test in four sera. According to the Hepanostika test 43 sera contained antibodies against HBs antigen, while 45 did according to the Ausab test. The two that were positive only in the Ausab test were only weakly positive.