Aberrant uterine natural killer (NK)-cell expression and altered placental and serum levels of the NK-cell promoting cytokine interleukin-12 in pre-eclampsia
Aberrant uterine natural killer (NK)-cell expression and altered placental and serum levels of the NK-cell promoting cytokine interleukin-12 in pre-eclampsia
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DOI：
10.1111/j.1600-0897.2006.00429.x
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发表时间：
2006-11-01
影响因子：
3.6
通讯作者：
Sverremark-Ekstrom, Eva
中科院分区：
文献类型：
--
作者：
Bachmayer, Nora;Hamad, Rangeen Rafik;Sverremark-Ekstrom, Eva
自然杀伤（NK）细胞是母胎界面最丰富的淋巴细胞群。它们在胎盘形成过程中通过控制滋养层侵入而发挥重要作用。通过免疫组化染色检测46名妇女、22名先兆子痫妇女和24名健康对照者的蜕膜NK（dNK）细胞。此外，我们研究了胎盘表达和血清水平的NK细胞活化细胞因子白细胞介素（IL）-12，IL-15，IL-18和抗炎细胞因子IL-10的IHC和酶联免疫吸附试验（ELISA）分别。先兆子痫妇女有较高的数量的CD 56（+）和CD 94（+）细胞在蜕膜中，表明dNK细胞的受体表达的改变。我们还首次证明，绒毛滋养层细胞在健康对照胎盘中显示出强烈的IL-12免疫染色，而患有先兆子痫的妇女的IL-12显著减少。然而，先兆子痫妇女血清中IL-12和IL-15水平显著升高，结果显示先兆子痫患者dNK细胞数量增加，表型改变，支持这些细胞对健康妊娠的重要性。dNK细胞受体表达的改变以及胎盘IL-12表达的减少可能涉及先兆子痫中NK细胞调节的改变。
Natural killer (NK) cells are the most abundant lymphocyte population at the maternal-fetal interface. They are suggested to be important during placentation by controlling trophoblast invasion. If placentation is suboptimal, pre-eclampsia can occur.Decidual NK (dNK) cells were examined at delivery in 46 women, 22 pre-eclamptic women and 24 healthy controls, by staining for CD56 and CD94 with immunohistochemistry (IHC). Furthermore, we investigated the placental expression and the serum levels of the NK-cell activating cytokines interleukin(IL)-12, IL-15, IL-18 and the anti-inflammatory cytokine IL-10 by IHC and enzyme-linked immunosorbent assay (ELISA), respectively.Pre-eclamptic women had higher number of CD56(+) and CD94(+) cells in the decidua, indicating an altered receptor expression of dNK cells. We also demonstrate for the first time that the villous trophoblasts show strong immunostaining for IL-12 in placentae from healthy controls, while women suffering from pre-eclampsia have significantly less IL-12. However, pre-eclamptic women had significantly elevated IL-12 and IL-15 levels in serum.Results show increased numbers and altered phenotype of dNK cells in pre-eclampsia, supporting the importance of these cells for a healthy pregnancy. The altered receptor expression of dNK cells together with diminished placental IL-12 expression could implicate an altered NK cell-regulation in pre-eclampsia.