Sea urchin (Lytechinus pictus) late-stage histone H3 and H4 genes: Characterization and mapping of a clustered but nontandemly linked multigene family
Sea urchin (Lytechinus pictus) late-stage histone H3 and H4 genes: Characterization and mapping of a clustered but nontandemly linked multigene family
复制标题
海胆 (Lytechinus pictus) 晚期组蛋白 H3 和 H4 基因：聚集但非串联连锁的多基因家族的表征和作图
DOI：
10.1016/0092-8674(82)90132-5
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发表时间：
1982
期刊：
影响因子：
64.5
通讯作者：
J. Knowles
中科院分区：
文献类型：
--
作者：
G. Childs;C. Nocente;T. Lieber;C. Holt;J. Knowles
我们克隆并鉴定了一个小型多基因家族的成员，该家族编码来自海胆 Lytechinus pictus 的晚期组蛋白 H3 和 H4 mRNA。与在早期发育阶段表达的高度重复组蛋白基因对应物不同，晚期 H3 和 H4 组蛋白基因不存在于串联重复中。此外，晚期H3和H4基因并不总是与早期组蛋白基因中的H1、HZA和HZB基因紧密地聚集在一起。分隔相邻 H3 和 H4 编码区的间隔 DNA 在晚期组蛋白基因家族的非等位基因成员之间并不保守。我们已经确定了连续 21 DO bp DNA 片段的核苷酸序列，包括 H3 和 H4 编码序列、分隔基因和周围非组蛋白 DNA 的整个间隔 DNA。晚期组蛋白 H3 和 H4 基因编码与其早期基因对应物相同的蛋白质；然而，晚期基因的 5' 前导序列较短，并且密码子的使用也不同。
We have cloned and characterized members of a small multigene family that encodes late-stage histone H3 and H4 mRNAs from the sea urchin Lytechinus pictus. Unlike their highly repetitive histone gene counterparts, which are expressed at an earlier developmental stage, late H3 and H4 histone genes are not present in tandem repeats. In addition, the late stage H3 and H4 genes are not always tightly clustered together with the Hl, HZA and HZB genes as they are in early histone genes. The spacer DNA that separates adjoining H3 and H4 coding regions is not conserved between nonallelic members of the late histone gene family. We have determined the nucleotide sequence of a continuous 21 DO bp segment of DNA including both H3 and H4 coding sequences, the entire spacer DNA separating the genes and surrounding nonhistone DNA. The late histone H3 and H4 genes encode proteins identical to their early gene counterparts; however, the 5’leader sequence is shorter in late genes and the codon usage is different.