Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection
Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection
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DOI：
10.1086/520088
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发表时间：
2007-09-01
影响因子：
6.4
通讯作者：
Gill, Steven R.
中科院分区：
文献类型：
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作者：
Fowler, Vance G., Jr.;Nelson, Charlotte L.;Gill, Steven R.
背景细菌克隆性对金黄色葡萄球菌引起的感染的影响尚不清楚。三百七十九S。金黄色葡萄球菌分离株（125株耐甲氧西林S.金黄色葡萄球菌[MRSA]和254株甲氧西林敏感的S.金黄色葡萄球菌[MSSA]）进行基因分型。对于MRSA分离株，还对葡萄球菌染色体盒mec（SCCmec）元件进行了分型。临床上分为三类：单纯鼻携带（n = 118）、单纯感染（n = 104）和菌血症伴血行并发症（n = 157）。利用eBURST，在371株分离株中发现18个克隆复合体（CC）。8个CC占分离株的89%，发生在所有临床类别中。CC 5（P = 0.0025）和CC 30（P = 0.0308）显示出更频繁的血液并发症的显著趋势。SPA 2型和16型内的分离株表现出相同的显著趋势，并分别聚在CC 5和CC 30内。SCCmec II型菌株与SCCmec IV型菌株相比也表现出同样的显著趋势; 96%的菌株为CC 5或CC 30。虽然大多数S。金黄色葡萄球菌基因型表现出导致侵袭性疾病的能力，CC 5和CC 30内的菌株表现出明显的增加血源性并发症水平的趋势。使用spa和SCCmec分型也涉及这些CC中的分离株。这些发现背后的遗传决定因素仍有待证明。
Background. The impact of bacterial clonality on infections caused by Staphylococcus aureus is unclear.Methods. Three hundred seventy-nine S. aureus isolates (125 methicillin-resistant S. aureus [MRSA] and 254 methicillin-susceptible S. aureus [MSSA]) were genotyped by spa typing and multilocus sequence typing. For MRSA isolates, the staphylococcal chromosomal cassette mec (SCCmec) element was also typed. Three clinical categories were identified: nasal carriage only (n = 118), uncomplicated infection (n = 104), and bacteremia with hematogenous complications (n = 157).Results. By use of eBURST, 18 clonal complexes (CCs) were found in 371 isolates. Eight CCs accounted for 89% of isolates and occurred in all clinical categories. CC5 (P = .0025) and CC30 (P = .0308) exhibited a significant trend toward more frequent hematogenous complications. Isolates within spa types 2 and 16 showed the same significant trend and grouped within CC5 and CC30, respectively. SCCmec II isolates also showed the same significant trend compared with SCCmec IV; 96% were CC5 or CC30.Conclusions. Although most S. aureus genotypes exhibited the capacity to cause invasive disease, strains within CC5 and CC30 exhibited a significant trend toward increasing levels of hematogenous complications. Isolates within these CCs were also implicated by use of spa and SCCmec typing. The genetic determinants underlying these findings remain to be demonstrated.