Characterization of damaged skin by impedance spectroscopy: chemical damage by dimethyl sulfoxide.
Characterization of damaged skin by impedance spectroscopy: chemical damage by dimethyl sulfoxide.
复制标题
通过阻抗谱表征受损皮肤：二甲亚砜造成的化学损伤。
DOI：
10.1007/s11095-013-1087-3
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发表时间：
2013
影响因子：
3.7
通讯作者：
Bunge,AnnetteL
中科院分区：
文献类型：
--
作者：
White,ErickA;Orazem,MarkE;Bunge,AnnetteL
目的探讨二甲基亚砜(DMSO)致皮肤损伤过程中电化学阻抗谱的变化及其与皮肤损伤机制的关系。方法将经DMSO或磷酸盐缓冲盐水(对照组)处理1h及以下的人身体皮肤前后的电化学阻抗谱与描述DMSO损伤进展的两种不同理论的电路模型进行比较。结果DMSO处理1h前后的阻抗谱符合单回路模型，而DMSO处理0.25h后的阻抗谱与串联处理1h前后的模型回路一致。与对照组相比，DMSO处理对对氯硝基苯的通量没有显著影响。结论人体皮肤暴露于DMSO小于0.5h时的阻抗测量与两层膜的存在相一致：一层是不可逆的，另一层是不变的。损伤层的厚度与治疗时间的平方根成正比，直到大约0.5小时，DMSO影响到整个角质层。不可逆的DMSO损伤对亲脂性渗透途径的影响最小。
PurposeTo relate changes in the electrochemical impedance spectra to the progression and mechanism of skin damage arising from exposure to dimethyl sulfoxide (DMSO).MethodsElectrochemical impedance spectra measured before and after human cadaver skin was treated with neat DMSO or phosphate buffered saline (control) for 1 h or less were compared with electrical circuit models representing two contrasting theories describing the progression of DMSO damage. Flux of a model lipophilic compound (p-chloronitrobenzene) was also measured.ResultsThe impedance spectra collected before and after 1 h treatment with DMSO were consistent with a single circuit model; whereas, the spectra collected after DMSO exposure for 0.25 h were consistent with the model circuits observed before and after DMSO treatment for 1 h combined in series. DMSO treatments did not significantly change the flux of p-chloronitrobenzene compared to control.ConclusionsImpedance measurements of human skin exposed to DMSO for less than about 0.5 h were consistent with the presence of two layers: one damaged irreversibly and one unchanged. The thickness of the damaged layer increased proportional to the square-root of treatment time until about 0.5 h, when DMSO affected the entire stratum corneum. Irreversible DMSO damage altered the lipophilic permeation pathway minimally.