Assay conditions can influence the outcome of cytotoxicity tests of nanomaterials: Better assay characterization is needed to compare studies
Assay conditions can influence the outcome of cytotoxicity tests of nanomaterials: Better assay characterization is needed to compare studies
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DOI：
10.1016/j.tiv.2009.10.007
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发表时间：
2010-03-01
影响因子：
3.2
通讯作者：
Hoet, Peter H. M.
中科院分区：
文献类型：
--
作者：
Geys, Jorina;Nemery, Benoit;Hoet, Peter H. M.
背景：在高通量规模上生成一致的纳米材料毒性数据的可靠体外研究将在未来几年中具有不可估量的意义。目的：在本研究中，我们检查了实验设计的几个步骤对结果的影响：我们研究了细胞密度、活力测定和颗粒分散方法的作用，包括血清的影响和表面活性剂（Tween 80）的影响。方法：评估研磨多壁碳纳米管的剂量反应曲线(CNT) 和二氧化硅基准 Min-U-Sil 对肺上皮细胞（A549 细胞）和巨噬细胞（刺激的 THP-1 细胞）的影响。结果：细胞毒性研究中使用的细胞密度对结果有影响：对于相同浓度的 Min-U-Sil，A549 细胞的活力随着细胞密度的增加从 10% 变化到 55%。虽然胎牛血清减弱了 Min-U-Sil 的细胞毒性，但 CNT 并未观察到这种效应。结果显示了细胞培养条件如何改变毒理学实验的结果，如本研究中使用 Tween 80 分散测试剂所显示的那样。结论：这些实验表明，除了使用基准颗粒之外，只有在提供详细的方法描述的情况下，才能对文献中报告的结果进行比较。因此，需要更加重视细胞毒性研究的标准化设计。 (C) 2009 Elsevier Ltd. 保留所有权利。
Background: Reliable in vitro studies that generate consistent toxicity data on nanomaterials on a high-throughput scale will be of invaluable significance in the next few years.Aim: In this study, we checked the influence of several steps of the experimental design on the outcome: we investigated the role of cell density, viability assay and particle dispersion method, including the influence of serum and effect of a surfactant (Tween 80).Methods: The dose-response curve was assessed for ground multiwalled carbon nanotubes (CNT) and the silica benchmark Min-U-Sil, on lung epithelial cells (A549 cells) and macrophages (stimulated THP-1 cells).Results: The cell density used in the cytotoxicity study has an impact on the outcome: for the same concentration of Min-U-Sil, the viability of A549 cells varied from 10% to 55% with increasing cell density. Whereas foetal calf serum attenuated the cytotoxicity of Min-U-Sil, this effect was not seen for CNT. The results show how cell culture conditions can modify the outcome of a toxicological experiment, as shown in this study for Tween 80 to disperse the test agent.Conclusions: These experiments illustrates that results reported in literature can only be compared when, in addition to the use of a benchmark particle, a detailed method description is available. Therefore, more emphasis is needed on a standardized design for cytotoxicity studies. (C) 2009 Elsevier Ltd. All rights reserved.