Anti-inflammatory cytokine profile in early human tendon repair
Anti-inflammatory cytokine profile in early human tendon repair
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DOI：
10.1007/s00167-012-2197-x
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发表时间：
2013-08-01
影响因子：
3.8
通讯作者：
Nader, G. A.
中科院分区：
文献类型：
--
作者：
Ackermann, P. W.;Domeij-Arverud, E.;Nader, G. A.
本研究的目的是评估炎症以及细胞因子的存在和相对水平，这些细胞因子可能参与调节人类早期跟腱愈合。纳入了 9 名急性跟腱断裂患者，并进行了手术和术后固定。术后两周，在愈合和完整的对侧跟腱中进行肌腱周围间质室的微透析。使用细胞珠阵列对肿瘤坏死因子 (TNF)-α、干扰素 (IFN)-γ、白细胞介素 (IL)-1 β、IL-6、IL-8、IL-10、IL-12p70 和 IL-17A 进行定量。通过酶免疫测定法测量前列腺素 (PG) E-2 水平。没有患者表现出可检测到的 PGE(2) 水平。促炎细胞因子低于检测水平（IFN γ、IL-12 和 IL-17），或者受伤肌腱和对照肌腱（IL-1 β 和 TNF）之间没有差异。值得注意的是，愈合中跟腱中的 IL-6、IL-8 和 IL-10 浓度显着升高：分别为 13 倍（p = 0.009）、28 倍（p = 0.02）和 3.7 倍（p = 0.03）。人类跟腱断裂后 2 周，愈合的特点是炎症阶段消退以及 IL-6、IL-8 和 IL-6 上调。 IL-10。没有炎症表明在这个时间点，这些细胞因子可能与肌腱愈合过程的抗炎和再生作用有关。
The aim of this study was to assess inflammation and the presence and relative levels of cytokines, which may be involved in regulating early human Achilles tendon healing.Nine patients with acute Achilles tendon rupture were included, operated on and post-operatively immobilized. Two weeks post-operatively, microdialysis of the peritendinous interstitial compartment was performed in the healing and intact contralateral Achilles tendons. Quantification of tumour necrosis factor (TNF)-alpha, interferon (IFN)-gamma, interleukin (IL)-1 beta, IL-6, IL-8, IL-10, IL-12p70 and IL-17A was accomplished using a cytometric bead array. Prostaglandin (PG) E-2 levels were measured by enzyme immunoassay.None of the patients displayed detectable PGE(2) levels. Pro-inflammatory cytokines were below detection levels (IFN gamma, IL-12, and IL-17) or did not differ between injured and control tendons (IL-1 beta and TNF). Notably, IL-6, IL-8 and IL-10 concentrations in the healing Achilles tendon were significantly elevated: 13-fold (p = 0.009), 28-fold (p = 0.02), and 3.7-fold (p = 0.03), respectively.At 2 weeks post-human Achilles tendon rupture, healing is characterized by a resolving inflammatory phase and up-regulation of IL-6, IL-8 and IL-10. The absence of inflammation suggests that at this time point, these cytokines may be associated with anti-inflammatory and regenerative effects on the tendon healing process.