Glutathione peroxidase levels throughout normal pregnancy and in pre-eclampsia.
Glutathione peroxidase levels throughout normal pregnancy and in pre-eclampsia.
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正常妊娠和先兆子痫期间的谷胱甘肽过氧化物酶水平。
DOI：
10.1080/jmf.12.5.322.326
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Kuczynski,E
中科院分区：
文献类型：
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作者：
Funai,EF;MacKenzie,A;Kadner,SS;Roque,H;Lee,MJ;Kuczynski,E
有证据表明，血红蛋白除了作为氧气载体的功能外，还可以将一氧化氮（如S-亚硝基半胱氨酸）从肺部运输到外周循环，并在外周循环中释放。谷胱甘肽过氧化物酶除了是一种重要的抗氧化剂外，还可以催化一氧化氮从较小的载体分子中释放出来，并可能在一氧化氮在全身的分布中发挥作用。根据这些研究结果，我们试图确定是否谷胱甘肽过氧化物酶水平在整个妊娠期不同，特别是先兆子痫和正常women.MethodsA巢式病例对照研究的妇女接受常规产前护理进行。先兆子痫定义为收缩压至少为140 mmHg和/或舒张压90 mmHg，以及蛋白尿> 300 mg/24 h或> 2+（通过试纸测定），均发生在两次至少间隔6 h的情况下。将血液收集在肝素化试管中，然后在临床离心机中离心10 min。血浆在-80 ° C下迅速冷冻，用于随后的酶联免疫吸附测定（ELISA），其中血浆谷胱甘肽过氧化物酶被测定。分娩时的胎次和胎龄。母亲的中位年龄分别为33岁和34岁，出生时的中位孕龄分别为37.5周和38.1周。在评估所有患者在三个孕期的谷胱甘肽过氧化物酶水平，我们发现，基本上没有差异的平均水平（83.7，81.0和89.5 ng/ml，分别）。再次按妊娠期分层，先兆子痫和非先兆子痫患者之间没有差异。线性回归分析表明，血浆谷胱甘肽过氧化物酶的浓度与孕龄或存在的先兆子痫。ConclusionsPlasma谷胱甘肽过氧化物酶的表达是相似的所有妊娠期。子痫前期患者谷胱甘肽过氧化物酶水平无变化。
ObjectiveEvidence suggests that hemoglobin, in addition to its function as a carrier of oxygen, also serves to transport nitric oxide, as S-nitroso cysteine, from the lungs to the peripheral circulation, where it can be released. Glutathione peroxidase, besides being an important antioxidant, is known to catalyze the release of nitric oxide from smaller carrier molecules, and may play a role in the distribution of nitric oxide throughout the body. In light of these findings, we sought to determine whether glutathione peroxidase levels differed throughout gestation, and specifically between pre-eclamptic and normal women.MethodsA nested case-control study of women receiving routine prenatal care was conducted. Pre-eclampsia was defined by a blood pressure of at least 140 mmHg systolic and/or 90 mmHg diastolic as well as proteinuria > 300 mg/24 h or > 2+ by dipstick, both occurring on two occasions at least 6 h apart. Blood was collected in heparinized tubes and was then centrifuged in a clinical centrifuge for 10 min. Plasma was frozen promptly at -80°C for later enzyme-linked immunosorbent assay (ELISA), with which plasma glutathione peroxidase was determined.ResultsThe maternal demographics of the pre-eclamptic and non-pre-eclamptic study groups did not significantly vary with respect to mean maternal age, gravidity, parity and gestational age at the time of delivery. The median maternal ages were 33 and 34 years, and the median gestational ages at the time of birth were 37.5 and 38.1 weeks, respectively. In evaluating the glutathione peroxidase levels of all patients across the three trimesters, we found that there was essentially no difference in mean levels (83.7, 81.0 and 89.5 ng/ml, respectively). There was no difference between the pre-eclamptic and non-pre-eclamptic patients, again stratified by trimester. A linear regression analysis indicated that the plasma glutathione peroxidase concentration did not correlate with gestational age or the presence of pre-eclampsia.ConclusionsPlasma glutathione peroxidase expression is similar across all trimesters. There is no change in the glutathione peroxidase levels in pre-eclamptic patients.