Intestinal Microbiota in Patients with Spinal Cord Injury.
Intestinal Microbiota in Patients with Spinal Cord Injury.
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DOI：
10.1371/journal.pone.0145878
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Gursel M
中科院分区：
文献类型：
--
作者：
Gungor B;Adiguzel E;Gursel I;Yilmaz B;Gursel M
人类肠道植物群包括数千种细菌。肠道微生物群的生长和组成取决于各种参数，包括免疫机制，饮食因素和肠道运动。脊髓损伤（SCI）患者经常表现出神经源性肠功能障碍，这是由于缺乏中枢神经系统对胃肠系统的控制。考虑到SCI患者的肠道功能障碍和结肠通过时间的改变，我们假设他们的肠道微生物组的组成发生了显著变化。本研究的目的是描述具有不同类型肠功能障碍的成年SCI患者的肠道微生物群。我们使用16S rRNA测序对30名SCI患者（15名上运动神经元[UMN]肠综合征，15名下运动神经元[LMN]肠综合征）和10名健康对照者进行了测试。从粪便中取样肠道微生物模式。独立于研究组，参与者的肠道微生物群以布劳特氏菌属、双歧杆菌属、粪杆菌属和瘤胃球菌属为主。当我们比较所有研究组时，罗斯拜瑞氏菌属、假丁酸弧菌属、小类杆菌属、Marvinbryantia和巨单胞菌属出现为组间统计学差异的属。与健康组相比，UMN肠功能障碍组的假丁酸弧菌属、小杆菌属和巨单胞菌属的细菌总数显著降低。与LMN组相比，UMN肠功能障碍组的Marvinbryantia属细菌总数显著降低。与健康组相比，LMN肠功能障碍组的罗斯拜瑞氏菌属、假丁酸弧菌属和巨单胞菌属的细菌总数显著降低。我们的研究结果首次表明，与健康受试者相比，SCI患者的丁酸盐生成成员特别减少。这项研究的结果将是有趣的，因为据我们所知，针对SCI患者的微生物组相关研究是不存在的，结果可能有助于解释SCI中肠道微生物组的可能影响。
Human intestinal flora comprises thousands of bacterial species. Growth and composition of intestinal microbiota is dependent on various parameters, including immune mechanisms, dietary factors and intestinal motility. Patients with spinal cord injury (SCI) frequently display neurogenic bowel dysfunction due to the absence of central nervous system control over the gastrointestinal system. Considering the bowel dysfunction and altered colonic transit time in patients with SCI, we hypothesized the presence of a significant change in the composition of their gut microbiome. The objective of this study was to characterize the gut microbiota in adult SCI patients with different types of bowel dysfunction. We tested our hypothesis on 30 SCI patients (15 upper motor neuron [UMN] bowel syndrome, 15 lower motor neuron [LMN] bowel syndrome) and 10 healthy controls using the 16S rRNA sequencing. Gut microbial patterns were sampled from feces. Independent of study groups, gut microbiota of the participants were dominated by Blautia, Bifidobacterium, Faecalibacterium and Ruminococcus. When we compared all study groups, Roseburia, Pseudobutyrivibrio, Dialister, Marvinbryantia and Megamonas appeared as the genera that were statistically different between groups. In comparison to the healthy group, total bacterial counts of Pseudobutyrivibrio, Dialister and Megamonas genera were significantly lower in UMN bowel dysfunction group. The total bacterial count of Marvinbryantia genus was significantly lower in UMN bowel dysfunction group when compared to the LMN group. Total bacterial counts of Roseburia, Pseudobutyrivibrio and Megamonas genera were significantly lower in LMN bowel dysfunction group when compared to healthy groups. Our results demonstrate for the first time that butyrate-producing members are specifically reduced in SCI patients when compared to healthy subjects. The results of this study would be of interest since to our knowledge, microbiome-associated studies targeting SCI patients are non-existent and the results might help explain possible implications of gut microbiome in SCI.