Isolation and characterization of a biologically active chromium oligopeptide from bovine liver
Isolation and characterization of a biologically active chromium oligopeptide from bovine liver
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DOI：
10.1006/abbi.1997.9878
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发表时间：
1997-03-15
影响因子：
3.9
通讯作者：
Vincent, JB
中科院分区：
文献类型：
--
作者：
Davis, CM;Vincent, JB
低分子铬结合物质(LMWCR)是已知的唯一一种天然存在于哺乳动物中的含铬多肽，也是铬的生物活性形式的候选者，首次以足够的产率被分离出来，能够在分子水平上提供结构和机制数据。顺磁H-1核磁共振、电子顺磁共振和电子光谱研究的结果表明，每个多肽的四个铬离子可能以整数自旋四核组装的形式排列，但也不能排除存在两个双核组装的可能性。该组件由阴离子配体桥联，并由多肽提供的羧酸盐支撑。(C)1997年学术出版社。
Low-molecular-weight chromium-binding substance (LMWCr), the only known naturally occurring Cr containing polypeptide from mammals and candidate for the biologically active form of chromium, has been isolated for the first time in yields sufficient for spectroscopic studies capable of providing structural and mechanistic data on a molecular level. The results of paramagnetic H-1 NMR, electron paramagnetic resonance, and electronic spectroscopic studies indicate that the four chromic ions per polypeptide are probably arranged in an integer-spin tetranuclear assembly, although an alternate possibility, the presence of two dinuclear assemblies, could not be ruled out. This assembly (or assemblies) is bridged by anionic ligands and supported by carboxylates provided by the polypeptide. (C) 1997 Academic Press.