The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range
The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range
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DOI：
10.1023/a:1026438615520
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发表时间：
1999-05-01
影响因子：
5.1
通讯作者：
Pakrasi, HB
中科院分区：
文献类型：
--
作者：
Korshunova, YO;Eide, D;Pakrasi, HB
锰在植物细胞中跨膜转运的分子基础知之甚少。我们已经发现，IRT1，拟南芥金属离子转运蛋白，可以补充突变体酿酒酵母菌株缺陷的高亲和力锰吸收（smf1三角洲）。IRT1蛋白先前已被鉴定为铁转运蛋白。目前的研究表明，IRT1在酵母中表达时，也可以转运锰。这种锰吸收活性被镉，铁（II）和锌抑制，表明IRT1可以运输这些金属。IRT1 cDNA还补充了锌摄取缺陷的酵母突变株（zrt1zrt2），并且镉、铜、钴和铁（III）抑制了酵母细胞中IRT1依赖的锌转运。然而，IRT1没有补充铜摄取缺陷的酵母突变体（ctr1），这意味着该转运蛋白不参与植物细胞中铜的摄取。IRT1在A.在缺铁条件下生长的植物。在这些条件下，根相关的锰，锌和钴的水平增加，这表明，除了铁，IRT 1介导这些金属进入植物细胞的吸收。综上所述，这些数据表明IRT 1蛋白是植物中的宽范围金属离子转运蛋白。
The molecular basis for the transport of manganese across membranes in plant cells is poorly understood. We have found that IRT1, an Arabidopsis thaliana metal ion transporter, can complement a mutant Saccharomyces cerevisiae strain defective in high-affinity manganese uptake (smf1 Delta). The IRT1 protein has previously been identified as an iron transporter. The current studies demonstrated that IRT1, when expressed in yeast, can transport manganese as well. This manganese uptake activity was inhibited by cadmium, iron(II) and zinc, suggesting that IRT1 can transport these metals. The IRT1 cDNA also complements a zinc uptake-deficient yeast mutant strain (zrt1zrt2), and IRT1-dependent zinc transport in yeast cells is inhibited by cadmium, copper, cobalt and iron(III). However, IRT1 did not complement a copper uptake-deficient yeast mutant (ctr1), implying that this transporter is not involved in the uptake of copper in plant cells. The expression of IRT1 is enhanced in A. thaliana plants grown under iron deficiency. Under these conditions, there were increased levels of root-associated manganese, zinc and cobalt, suggesting that, in addition to iron, IRT1 mediates uptake of these metals into plant cells. Taken together, these data indicate that the IRT1 protein is a broad-range metal ion transporter in plants.