Calorie restriction inhibits relapse behaviour and preference for alcohol within a two-bottle free choice paradigm in the alcohol preferring (iP) rat
Calorie restriction inhibits relapse behaviour and preference for alcohol within a two-bottle free choice paradigm in the alcohol preferring (iP) rat
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DOI：
10.1016/j.physbeh.2012.11.011
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发表时间：
2013-02-17
影响因子：
2.9
通讯作者：
Paolini, Antonio G.
中科院分区：
文献类型：
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作者：
Guccione, Lisa;Djouma, Elvan;Paolini, Antonio G.
在许多有益的作用中，卡路里限制(CR)也被发现可以减少啮齿类动物的焦虑相关行为。随着压力和焦虑水平的增加被认为是复发和酒精成瘾的关键诱发因素，研究发现，25%的CR除了诱导焦虑作用外，还在手术自我给药模型中具有减少酒精摄入量和抑制复发的能力。这项研究的目的是调查在两瓶免费选择范例中，25%的CR是否也会显示出类似的效果，其中24人可以同时获得10%的乙醇和水。所有动物在开始两瓶自由选择范式之前，首先在高架加迷宫(EPM)和开场测试中进行测试。对照组和CR25%组动物之间的差异显示了CR的抗焦虑作用，CR25%组在EPM中显示出更高的张开手臂/总手臂持续时间百分比和开放手臂/总手臂条目百分比。在两瓶自由选择范例的习得阶段，与对照组相比，CR25%的动物显示出以毫升/公斤为单位的10%乙醇的摄入量减少。虽然对照组动物表现出强烈的10%乙醇偏好，但CR25%组摄入10%乙醇和水的比例相同，两组之间的总液体摄入量没有差异。同样，强迫剥夺之后的情况也是如此。除了摄入减少和对10%乙醇缺乏偏好外，CR25%的动物与对照组不同，在禁欲后没有表现出典型的酒精剥夺效应。综上所述，这项研究的结果表明，CR可能作为一种保护因素，防止成瘾和复发的酒精偏爱(IP)大鼠。此外，考虑到CR25%的动物没有表现出对10%乙醇的偏好，结果也表明CR可能改变了这一组中乙醇的享乐影响。(C)2012 Elsevier Inc.保留所有权利。
Among its many beneficial effects, calorie restriction (CR) has also been found to reduce anxiety related behavior in the rodent. With heightened levels of stress and anxiety implicated as a key precipitating factor of relapse and alcohol addiction, it was found that a 25% CR in addition to inducing anxiolytic effects also had the capacity to reduce intake of alcohol and inhibit relapse within a model of operant self-administration. The aim of this study was to investigate if a 25% CR would also display similar effects in a two-bottle free choice paradigm, whereby 24 had libitum access to both 10% ethanol and water is provided. All animals were initially tested on the elevated plus maze (EPM) and open field test prior to commencing the two-bottle free choice paradigm. Differences between control and CR25% animals demonstrated the anxiolytic effects of CR, with the CR25% group displaying greater percentage of open arm/total arm duration and open arm/total arm entries in the EPM. During the acquisition phase of the two-bottle free choice paradigm, CR25% animals showed a reduced intake of 10% ethanol in ml/kg, in comparison to the control group. Whilst control animals displayed a strong preference for 10% ethanol, the CR25% group consumed both 10% ethanol and water equally with no differences found in total fluid intake between groups. Similarly this was also the case following forced deprivation. In addition to reduced intake and lack of preference for 10% ethanol, CR 25% animals unlike controls failed to display a typical alcohol deprivation effect following abstinence. Taken collectively the results of this study suggest that CR may act as a protective factor against addiction and relapse in the alcohol preferring (iP) rat. In addition, given CR25% animals did not display a preference for 10% ethanol, results also suggest that CR may be altering the hedonic impact of ethanol within this group. (c) 2012 Elsevier Inc. All rights reserved.