ADAPTATION OF BIOLOGICAL-MEMBRANES TO TEMPERATURE - EFFECT OF TEMPERATURE-ACCLIMATION OF GOLDFISH UPON VISCOSITY OF SYNAPTOSOMAL MEMBRANES
ADAPTATION OF BIOLOGICAL-MEMBRANES TO TEMPERATURE - EFFECT OF TEMPERATURE-ACCLIMATION OF GOLDFISH UPON VISCOSITY OF SYNAPTOSOMAL MEMBRANES
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DOI：
10.1016/0005-2736(77)90131-6
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
COSSINS, AR
中科院分区：
文献类型：
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作者：
COSSINS, AR
从金鱼中分离的突触体膜制备物的流动性适应于5、15和25度。以1，6-二苯基-1，3，5-己三烯为探针，用荧光偏振技术测定了大鼠体内的C和.冷驯化金鱼膜流体比那些温暖驯化金鱼在中间温度下测量时，表明温度依赖性调节这个参数。同样，膜的温暖驯化金鱼更流体比那些从大鼠大脑。从金鱼和大鼠突触体制备的纯化磷脂制备的脂质体显示出类似的天然膜的差异。冷驯化金鱼的膜流动性增加与主要磷脂类的饱和脂肪酸的比例下降和胆碱磷酸甘油酯的不饱和指数增加相关。与25 ℃的膜相比，大鼠膜显示出不饱和指数的显著降低和饱和脂肪酸比例的增加。C-驯化的金鱼。金鱼突触体膜胆固醇含量不受驯化处理的影响。本文还讨论了恒温动物在热驯化过程中对膜过程速率的补偿作用以及恒温动物对极端温度的抗性适应。
The fluidity of synaptosomal membrane preparations isolated from goldfish acclimated to 5, 15 and 25.degree. C and from rat was estimated using the fluorescence polarization technique with 1,6-diphenyl-1,3,5-hexatriene as probe. Membranes of cold-acclimated goldfish were more fluid than those of warm-acclimated goldfish when measured at an intermediate temperature, indicating a temperature-dependent regulation of this parameter. Similarly, membranes of warm-acclimated goldfish were more fluid than those prepared from rat brain. Liposomes prepared from the purified phospholipids of goldfish and rat synaptosomal preparations showed differences similar to those of the native membranes. Increased membrane fluidity of cold-acclimated goldfish was correlated with a decrease in the proportion of saturated fatty acids of the major phospholipid classes and an increased unsaturation index in choline phosphoglycerides. Rat membranes showed a substantial reduction in unsaturation index and an increase in the proportion of saturated fatty acids compared to the membranes of 25.degree. C-acclimated goldfish. The cholesterol content of synaptosomal membranes of goldfish was unaffected by acclimation treatment. The role of homeoviscous adaptation in the compensation of the rates of membrane processes during thermal acclimation, and on the resistance adaptation of poikilotherms to extreme temperatures is discussed.