Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique
Quantitative Assessment of Intestinal Injury Using a Novel In Vivo, Near-Infrared Imaging Technique
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DOI：
10.2310/7290.2010.00001
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Coimbra, Raul
中科院分区：
文献类型：
--
作者：
Costantini, Todd W.;Eliceiri, Brian P.;Coimbra, Raul
由炎症、严重创伤和烧伤引起的肠损伤是发病率和死亡率的主要原因。目前，用于研究肠道对损伤和炎症反应的动物模型依赖于过时的分析方法。鉴于这些经典的肠道试验对实验动物是致命的，因此无法研究同一动物随着时间的推移对损伤的肠道反应。我们推测，通过开发一种体内检测方法，使用荧光染料成像肠损伤，它可以补充其他昂贵，耗时，半定量的经典手段检测肠损伤。我们描述了一种新的在体内，非侵入性的方法来成像肠损伤使用电荷耦合器件（CCD）相机，允许串行视觉和定量分析肠损伤。我们的研究结果与传统的、耗时的、半定量的肠损伤检测相关，现在允许随着时间的推移对损伤进行非侵入性、非致命性的评估。
Intestinal injury owing to inflammation, severe trauma, and burn is a leading cause of morbidity and mortality. Currently, animal models employed to study the intestinal response to injury and inflammation depend on outdated methods of analysis. Given that these classic intestinal assays are lethal to the experimental animal, there is no ability to study the gut response to injury in the same animal over time. We postulated that by developing an in vivo assay to image intestinal injury using fluorescent dye, it could complement other expensive, time-consuming, and semiquantitative classic means of detecting intestinal injury. We describe a novel in vivo, noninvasive method to image intestinal injury using a charge-coupled device (CCD) camera that allows for serial visual and quantitative analysis of intestinal injury. Our results correlate with traditional, time-consuming, semiquantitative assays of intestinal injury, now allowing the noninvasive, nonlethal assessment of injury over time.