Highly Sensitive Closed Loop Enclosed Split Ring Biosensor With High Field Confinement for Aqueous and Blood-Glucose Measurements
Highly Sensitive Closed Loop Enclosed Split Ring Biosensor With High Field Confinement for Aqueous and Blood-Glucose Measurements
复制标题
高灵敏度闭环封闭式开口环生物传感器，具有高场限制，适用于水和血糖测量
DOI：
10.1038/s41598-020-60806-9
复制
发表时间：
2020-03-05
影响因子：
4.6
通讯作者：
Nie, Zedong
中科院分区：
文献类型：
--
作者：
Kandwal, Abhishek;Igbe, Tobore;Nie, Zedong
本文介绍了一种高灵敏度的闭环封闭的开口环生物传感器工作在微波频率测量人体血糖水平。该微波葡萄糖生物传感器基于高场限域和能量集中的原理，采用体外和体内两种方法进行了测试。该原理允许传感器将能量集中在表面，从而提高测量精度。对于在体外测量，生物传感器已被测试使用去离子水葡萄糖溶液的不同浓度。利用光刻技术在薄硅衬底上制作了微型化的微米级生物传感器。生物传感器已经被设计成在期望的微波频率下操作。高限制的领域和集中的能量内的闭环线包含分裂环谐振器负责的灵敏度提高。这种新的生物传感器在人体体内测试期间在临床糖尿病范围内获得了82 MHz/mgmL− 1的高灵敏度。此外，在整个实验过程中持续监测受试者（进行实验）的稳态，这有助于提高结果的准确性。所提出的生物传感器进一步获得了<0.05 wt.%的低检测限并且可用于连续非侵入性血糖监测。
This paper presents a highly sensitive closed loop enclosed split ring biosensor operating in microwave frequencies for measuring blood glucose levels in the human body. The proposed microwave glucose biosensor, working on the principle of high field confinement and concentrated energy, has been tested using bothin-vitroandin-vivomethods. This principle allows the sensor to concentrate energy at the surface which results in improved accuracy of measurements. Forin-vitromeasurements, the biosensor has been tested using de-ionized water glucose solutions of different concentrations. The miniaturized micrometer scale biosensor is fabricated over a thin Si-substrate using photolithographic technique. The biosensor has been designed in a way to operate at desired microwave frequencies. Highly confined fields and concentrated energy inside the closed loop line containing the split ring resonators are responsible for the sensitivity enhancement. This new biosensor has obtained a high sensitivity of 82 MHz/mgmL−1within the clinical diabetic range duringin-vivotesting over the human body. In addition, the subjects (undergoing experiments) steady state has been continuously monitored throughout the experiment which helps in improving the accuracy of the results. The proposed biosensor has further obtained a low detection limit of <0.05 wt.% and can be useful for continuous non-invasive blood glucose monitoring.