CDNA microarray analysis of nerve growth factor-regulated gene expression profile in rat PC12 cells
CDNA microarray analysis of nerve growth factor-regulated gene expression profile in rat PC12 cells
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DOI：
10.1007/s11064-005-2688-y
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Lee, EH
中科院分区：
文献类型：
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作者：
Lee, KH;Ryu, CJ;Lee, EH
神经生长因子 (NGF) 驱动的 PC12 细胞分化为神经元样细胞，为研究神经元分化过程提供了代表性的模型系统。尽管进行了广泛的研究，但与 PC12 细胞分化程序相关的基因调控仍然需要阐明。我们使用 cDNA 微阵列分析来表征 PC12 细胞对 NGF mRNA 表达的反应。 NGF 处理 5 天后，46 个基因受到 2 倍或更多的可重复影响。二十五个受调控的转录本与具有已知功能的基因相匹配。在通过实时逆转录酶测定证实的微阵列结果中，有几个基因以前未知受 NGF 调节。结果主要反映了调节神经可塑性、细胞骨架组织和脂质代谢的分子的变化，包括神经突蛋白、PDZ蛋白Mrt1、脂蛋白脂肪酶、原调节蛋白1和rhoB。这些观察到的基因变化可能提供有关 NGF 促进 PC12 细胞分化的分子机制的新信息。
Nerve growth factor (NGF)-driven differentiation of PC12 cells into neuronal-like cells provides a representative model system for studying neuronal differentiation processes. Despite of extensive research, gene regulation associated with the differentiation program in PC12 cells still needs to be elucidated. We used cDNA microarray analysis to characterize the response of PC12 cells to NGF at mRNA expression. Forty-six genes were reproducibly influenced by 2-fold or more after NGF treatment for 5 days. Twenty-five of the regulated transcripts were matched to genes which have known functions. Among the microarray results confirmed with real-time reverse transcriptase assay, several genes have not previously known to be modulated by NGF. The results mostly reflected changes in molecules regulating neural plasticity, cytoskeletal organization, and lipid metabolism, which include neuritin, PDZ protein Mrt1, lipoprotein lipase, tropomodulin 1 and rhoB. These observed genetic changes may provide new information about molecular mechanisms underlying NGF-promoted differentiation of PC12 cells.