Carbon black particles increase reactive oxygen species formation in rat alveolar macrophages in vitro
Carbon black particles increase reactive oxygen species formation in rat alveolar macrophages in vitro
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DOI：
10.1007/s00204-006-0164-3
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发表时间：
2007-06-01
影响因子：
6.1
通讯作者：
Fonnum, F.
中科院分区：
文献类型：
--
作者：
Aam, Berit Bjugan;Fonnum, F.
肺泡巨噬细胞（AM）在清除肺部颗粒物方面具有重要作用。在不同的刺激下，它们可以释放活性氧（ROS）和炎症介质，促进肺部炎症。我们将大鼠AM暴露于炭黑（CB）颗粒（0.63-20 μ g/ml）中，并使用荧光探针2 '，7'-二氯荧光素二乙酸酯测量ROS的产生。在暴露于CB的AM中，荧光以浓度依赖性方式升高。使用一系列酶抑制剂的后续实验表明，ERK MAP激酶途径和p38 MAP激酶途径可能参与ROS的形成。
Alveolar macrophages (AM) have an important role in clearing particles from the lungs. In response to different stimuli they can release reactive oxygen species (ROS) and inflammatory mediators and promote pulmonary inflammation. We exposed rat AM to carbon black (CB) particles (0.63-20 mu g/ml) and measured the generation of ROS by using the fluorescent probe 2 ',7 '-dichlorofluorescein diacetate. Fluorescence was elevated in a concentration dependent manner in the AM exposed to CB. Follow-up experiments using a series of enzyme inhibitors indicate that the ERK MAP kinase pathway and the p38 MAP kinase pathway may be involved in the formation of ROS.