Oxygen therapy in permanent brain ischemia: Potential and limitations
Oxygen therapy in permanent brain ischemia: Potential and limitations
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DOI：
10.1016/j.brainres.2006.05.108
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发表时间：
2006-08-30
期刊：
影响因子：
2.9
通讯作者：
Schwaninger, Markus
中科院分区：
文献类型：
--
作者：
Veltkamp, Roland;Sun, Li;Schwaninger, Markus
背景：常压（NBO）和高压（HBO）氧疗对短暂性脑缺血都有保护作用。相比之下，在反映大多数临床中风的永久性缺血模型中，NBO 的有效性尚不清楚，而 HBO 的有效性也存在争议。本研究的目的是比较两种氧疗在两种永久性缺血模型中的作用，研究时间窗的效果，并评估两种氧疗的组合。方法：分别通过电凝或细丝诱导大脑中动脉（MCAO）远端或近端永久闭塞。小鼠接受空气、NBO、单次或重复的 HBO (3 ata) 治疗。分别在 7 天（凝固）和 24 小时（丝状）时对梗塞大小进行量化。结果：MCA 凝固后，呼吸空气的小鼠梗塞体积为 12.9 +/- 1.6 mm(3)。当 MCAO 后 45 分钟或 120 分钟开始使用时，NBO (10.8 +/- 2.2) 和更有效的 HBO (7.8 +/- 0.9) 可减少梗塞面积。重复 HBO 治疗没有额外效果 (8.3 +/- 2.3)。 HBO 还显着降低了 24 小时时的 TUNEL 细胞染色。 60 分钟 NBO 加 60 分钟 HBO 的组合比单独 120 分钟 NBO (11.1 +/- 3.2) 产生更小的皮质梗塞 (8.7 +/- 1.5)。相比之下，接受空气 (50 +/- 24 mm(3))、NBO (48 +/- 16) 或 HBO (46 +/- 21) 的啮齿动物之间，丝诱导的永久性 MCAO 的梗死体积没有差异。然而，在丝诱导短暂 MCAO 后，HBO 显着减少了梗塞体积。结论：NBO 和更有效的 HBO 可以保护大脑免受永久性皮质缺血。然而，在广泛的局灶性缺血中，氧疗仅在早期再通的情况下有效。 (c) 2006 Elsevier B.V. 保留所有权利。
Background: Both normobaric (NBO) and hyperbaric (HBO) oxygen therapy are protective in transient cerebral ischemia. In contrast, in permanent ischemia models, which reflect the majority of clinical strokes, the effectiveness of NBO is unknown, and the effectiveness of HBO is controversial. The goals of the present study were to compare both oxygen therapies in 2 models of permanent ischemia, to study the effect of time window, and to evaluate the combination of both oxygen therapies. Methods: Distal or proximal permanent occlusion of middle cerebral artery (MCAO) was induced by coagulation or filament, respectively. Mice received air, NBO, a single or repeated HBO (3 ata) treatments. Infarct sizes were quantified at 7 days (coagulation) and 24 h (filament), respectively. Results: Following MCA coagulation, infarct volume was 12.9 +/- 1.6 mm(3) in mice breathing air. When started 45 min or 120 min after MCAO, NBO (10.8 +/- 2.2) and significantly more potently HBO (7.8 +/- 0.9) reduced infarct size. Repeated HBO treatments had no additional effect (8.3 +/- 2.3). HBO also significantly decreased TUNEL cell staining at 24 h. Combination of 60 min NBO plus 60 min HBO resulted in smaller cortical infarcts (8.7 +/- 1.5) than 120 min NBO alone (11.1 +/- 3.2). In contrast, infarct volumes in filament-induced permanent MCAO did not differ among rodents receiving air (50 +/- 24 mm(3)), NBO (48 +/- 16), or HBO (46 +/- 21). After filament-induced transient MCAO, however, HBO reduced infarct volume significantly. Conclusions: NBO and more effectively HBO protect the brain against permanent cortical ischemia. In extensive focal ischemia, however, oxygen therapy is only effective in case of early recanalization. (c) 2006 Elsevier B.V. All rights reserved.