Pretreatment of raw glycerol with activated carbon for 1,3‐propanediol production by Clostridium butyricum
Pretreatment of raw glycerol with activated carbon for 1,3‐propanediol production by Clostridium butyricum
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DOI：
10.1002/elsc.201200106
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发表时间：
2013-07
影响因子：
2.7
通讯作者：
Chunjie Zhu;Bin Chen;B. Fang
中科院分区：
文献类型：
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作者：
Chunjie Zhu;Bin Chen;B. Fang
粗甘油含有抑制杂质，使其难以生物转化。采用活性炭对丁酸梭菌发酵1，3-丙二醇所用的粗甘油进行预处理。与纯甘油相比，未经处理的粗甘油抑制发酵。因此，在分批发酵中，采用粉状活性炭作为吸附剂处理粗甘油，使发酵时间从18.5 h缩短到13.3 h。采用均匀设计法考察了pH值、活性炭投加量和吸附时间对预处理效果的影响。结果表明，最佳预处理条件为：pH 3.0，粉末活性炭0.5g/150 g原料甘油，吸附时间3 h。在预处理的粗甘油上的分批培养与在纯甘油上的分批培养相当。补料分批培养中的1，3-丙二醇浓度在预处理的粗甘油上为49.3g/L，而在未处理的粗甘油上为40.8g/L。吸附前后活性炭的红外光谱分析表明，活性炭中的抑制杂质可能是芳香族化合物。这项工作表明，预处理是必要的发酵粗甘油1，3-丙二醇和预处理与粉尘活性炭是一种有效的，经济的，简单的方法。
Raw glycerol contains inhibiting impurities that render its bioconversion difficult. In this work, raw glycerol used in 1,3‐propanediol fermentation by Clostridium butyricum was pretreated with activated carbon. Compared with pure glycerol, the untreated raw glycerol inhibited fermentation. Therefore, the raw glycerol was treated using dusty activated carbon as an adsorbent in batch fermentation, resulting in a shortening of the fermentation time from 18.5 to 13.3 h. The effects of pH, dusty activated carbon amount, and adsorption time on the pretreatment results were investigated using uniform design. The pH had a much lower impact and the optimal pretreatment conditions were achieved: pH 3.0, 0.5 g dusty activated carbon/150 g raw glycerol, adsorption time 3 h. Batch cultures on pretreated raw glycerol were comparable to those on pure glycerol. The 1,3‐propanediol concentration in fed‐batch cultivation was 49.3 g/L on pretreated raw glycerol while it was 40.8 g/L on untreated raw glycerol. The FTIR spectra of dusty activated carbon before and after adsorption indicated that the inhibiting impurities were probably aromatic compounds. This work indicates that pretreatment is necessary to ferment raw glycerol to 1,3‐propanediol and that pretreatment with dusty activated carbon is an efficient, economical, and simple method.