Cloning of hexamethylene-bis-acetamide-inducible transcript, HEXIM1, in human vascular smooth muscle cells
Cloning of hexamethylene-bis-acetamide-inducible transcript, HEXIM1, in human vascular smooth muscle cells
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DOI：
10.2220/biomedres.20.273
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发表时间：
1999-10-01
影响因子：
1.2
通讯作者：
Yamaguchi, K
中科院分区：
文献类型：
--
作者：
Kusuhara, M;Nagasaki, K;Yamaguchi, K
血管平滑肌细胞（VSMC）的去分化和异常生长是血管疾病的重要组成部分。六亚甲基二乙酰胺（HMBA）在体内外均能调节血管平滑肌细胞的分化和生长。为探讨其作用机制，我们用HMBA处理人冠状动脉VSMC，并通过mRNA差异显示分析HMBA对基因表达的影响。HMBA调节了许多特定信息片段的表达。通过马拉松延伸和cDNA筛选，我们分离到一个新的基因HEXIM 1，该基因在HMBA刺激的VSMC中上调。HEXIM 1 cDNA全长3624 bp，编码359个氨基酸。北方印迹分析显示，HMBA刺激后1 h HEXIM 1 mRNA水平迅速升高，并持续48 h。这些结果表明HEXIM 1在调节VSMC的生长和分化中发挥重要作用。
The dedifferentiation and the abnormal growth of vascular smooth muscle cells (VSMC) is a major component of vascular diseases. Hexamethylene-bis-acetamide (HMBA) has recently been shown to modulate the differentiation and growth of VSMC in vitro and in vivo. To determine the mechanism of this effect, we treated human coronary artery VSMC with HMBA and analyzed the effect of HMBA on gene expression by mRNA differential display. HMBA modulated the expression of a number of specific message fragments. By Marathon extension and cDNA screening, we isolated a novel gene, HEXIM1, that is up-regulated in HMBA-stimulated VSMC. HEXIM1 cDNA has a length of 3624 bp and encodes for a protein of 359 amino acids. Northern blot analysis demonstrated that the level of HEXIM1 mRNA rapidly increased at 1 h after HMBA stimulation and was sustained for 48 h. These results suggest that HEXIM1 plays an important role in the regulation of the growth and differentiation of VSMC.