Dietary quality and the colonic mucosa-associated gut microbiome in humans
Dietary quality and the colonic mucosa-associated gut microbiome in humans
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DOI：
10.1093/ajcn/nqz139
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发表时间：
2019-09-01
影响因子：
7.1
通讯作者：
Jiao, Li
中科院分区：
文献类型：
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作者：
Liu, Yanhong;Ajami, Nadim J.;Jiao, Li
背景：尽管人们对调节微生物群以改善健康抱有极大兴趣，但健康个体中饮食与结肠粘膜相关肠道微生物群之间的关联尚未得到研究。目的：研究健康饮食指数 (HEI)-2005 与结肠粘膜相关微生物群之间的关联。方法：在这项横断面观察性研究中，我们使用 97 个结肠粘膜活检样本的 16S rRNA 基因（V4 区）测序分析了细菌群落组成和结构。通过内窥镜从 34 名无息肉参与者的不同结肠段获得。使用 FFQ 确定膳食消耗。比较总 HEI 及其组成部分的较高和较低分数之间的 α 和 β 多样性以及分类学相对丰度的差异，然后进行多变量分析。结果：总 HEI、总水果和整个水果（HEI 1 和 HEI 2）、全谷物（HEI 6）、奶制品和大豆饮料（HEI 7）以及固体脂肪、酒精和添加糖（HEI）的得分显着差异微生物群的结构12）。 HEI 总分以及 HEIs 2、7 和 12 得分较低与丰富度显着降低相关。总 HEI 得分较低与副拟杆菌属、罗斯伯里氏菌属和 Subdolicapsulum 的相对丰度显着降低有关，但梭杆菌属的相对丰度较高。 HEI 2 得分较低与罗斯氏菌属较低但拟杆菌属较高相关。 HEI 7 得分较低与粪杆菌属和梭杆菌属较低但拟杆菌属较高相关。 HEI 12 得分较低与 Subdoligrinum 较低但埃希氏菌和梭杆菌较高相关（错误发现率调整后的 P 值）
Background: Despite tremendous interest in modulating the microbiome to improve health, the association between diet and the colonic mucosa-associated gut microbiome in healthy individuals has not been examined.Objective: To investigate the associations between Healthy Eating Index (HEI)-2005 and the colonic mucosa-associated microbiota.Methods: In this cross-sectional observational study, we analyzed bacterial community composition and structure using 16S rRNA gene (V4 region) sequencing of 97 colonic mucosal biopsies obtained endoscopically from different colon segments of 34 polyp-free participants. Dietary consumption was ascertained using an FFQ. Differences in alpha- and beta-diversity and taxonomic relative abundances between the higher and lower score of total HEI and its components were compared, followed by multivariable analyses.Results: The structure of the microbiota significantly differed by the scores for total HEI, total and whole fruits (HEI 1 and HEI 2), whole grains (HEI 6), milk products and soy beverages (HEI 7), and solid fat, alcohol, and added sugar (HEI 12). A lower score for total HEI and HEIs 2, 7, and 12 was associated with significantly lower richness. A lower score for total HEI was associated with significantly reduced relative abundance of Parabacteroides, Roseburia, and Subdoligranulum but higher Fusobacterium. A lower score for HEI 2 was associated with lower Roseburia but higher Bacteroides. A lower score for HEI 7 was associated with lower Faecalibacterium and Fusobacterium but higher Bacteroides. A lower score for HEI 12 was associated with lower Subdoligranulum but higher Escherichia and Fusobacterium (false discovery rate-adjusted P values