Immune responses to bile-tolerant Helicobacter species in patients with chronic liver diseases, a randomized population group, and healthy blood donors
Immune responses to bile-tolerant Helicobacter species in patients with chronic liver diseases, a randomized population group, and healthy blood donors
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DOI：
10.1128/cdli.9.6.1160-1164.2002
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发表时间：
2002-11-01
期刊：
影响因子：
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通讯作者：
Wadström, T
中科院分区：
文献类型：
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作者：
Ananieva, O;Nilsson, I;Wadström, T
耐胆螺杆菌，如白痢螺杆菌、胆螺杆菌和肝螺杆菌，与动物肝脏疾病有关，并可能参与人类慢性肝病 (CLD) 的发病机制。使用酶联免疫吸附测定 (ELISA) 评估 CLD 患者、随机人群和健康献血者血清样本中对鸡白血球菌、胆汁菌和肝螺杆菌细胞表面蛋白的抗体反应。将结果与幽门螺杆菌的抗体反应进行比较。为了分析耐胆汁螺杆菌和幽门螺杆菌之间可能的交叉反应性，用幽门螺杆菌的全细胞提取物吸收来自每组亚群的血清，并通过 ELISA 重新测试。吸收前结果显示，慢性肺病患者的鸡白痢ELISA单位平均值显着高于健康献血者（P = 0.01）。 CLD 患者对肝螺杆菌细胞表面蛋白的抗体反应性也显着高于健康献血者和人群（分别为 P = 0.005 和 P = 0.002）。吸收后，所有三组中对白痢的抗体反应均显着下降（CLD 患者的 P = 0.0001，人群的 P = 0.0005，献血者的 P < 0.0001），表明幽门螺杆菌和其他螺杆菌之间的交叉反应性。发生。与吸收前的 ELISA 结果相比，CLD 患者在吸收实验后对肝螺杆菌和胆汁螺杆菌的抗体反应仍然很高。这一发现的意义需要进一步研究。
Bile-tolerant Helicobacter species such as Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of H. pullorum, H. bilis, and H. hepaticus in serum samples from patients with CLD, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (ELISA). The results were compared with the antibody responses to Helicobacter pylori. For analysis of a possible cross-reactivity between bile-tolerant Helicobacter species and H. pylori, sera from a subpopulation of each group were absorbed with a whole-cell extract of H. pylori and retested by ELISA. Results before absorption showed that the mean value of the ELISA units for H. pullorum was significantly higher in patients with CLD than in healthy blood donors (P = 0.01). Antibody reactivity to cell surface protein of H. hepaticus was also significantly higher in the CLD patients than in the healthy blood donors and the population group (P = 0.005 and P = 0.002, respectively). Following the absorption, antibody responses to H. pullorum decreased significantly in all three groups (P = 0.0001 for CLD patients, P = 0.0005 for the population group, and P < 0.0001 for the blood donors), indicating that cross-reactivity between H. pylori and other Helicobacter spp. occurs. The antibody responses to H. hepaticus and H. bilis in CLD patients remained high following absorption experiments compared to ELISA results before absorption. The significance of this finding requires further investigations.