Multiparametric real-time sensing of cytosolic physiology links hypoxia responses to mitochondrial electron transport.
Multiparametric real-time sensing of cytosolic physiology links hypoxia responses to mitochondrial electron transport.
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DOI：
10.1111/nph.16093
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发表时间：
2019-09
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通讯作者：
Stephan Wagner;Janina Steinbeck;Philippe Fuchs;Sophie Lichtenauer;Marlene Elsässer;Jos H. M. Schippers;Thomas Nietzel;Cristina Ruberti;Olivier Van Aken;A. Meyer;Joost T. van Dongen;Romy R. Schmidt;M. Schwarzländer
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作者：
Stephan Wagner;Janina Steinbeck;Philippe Fuchs;Sophie Lichtenauer;Marlene Elsässer;Jos H. M. Schippers;Thomas Nietzel;Cristina Ruberti;Olivier Van Aken;A. Meyer;Joost T. van Dongen;Romy R. Schmidt;M. Schwarzländer
缺氧在植物发育过程中经常发生，可以由环境引起，例如通过洪水。·为了了解植物组织生理学如何响应氧气限制，我们旨在实时和体内监测亚细胞生理学。我们建立了一个基于荧光蛋白传感器的拟南芥叶片亚细胞生理动态变化的多参数监测系统，并验证了其适用于缺氧胁迫。·通过平行监测MgATP 2-、游离Ca 2+浓度、pH、NAD氧化还原状态和谷胱甘肽氧化还原状态的细胞溶质动力学，与转录和代谢反应相关，我们生成了对进展性缺氧的生理反应的综合图像。我们表明，生理变化是令人惊讶的强大，即使当植物的碳状态被修改为实现蔗糖喂养或延长夜间。线粒体呼吸链的抑制导致细胞溶质生理学的动力学，这是非常相似的氧耗尽下，突出线粒体电子传递的细胞器以外的缺氧的细胞后果的关键决定因素。·建立了一个广泛适用的系统，用于植物胁迫生理学的并行体内传感，以绘制出线粒体逆行信号和低氧信号发生的生理背景，表明共享的上游刺激。本文受版权保护。All rights reserved.
•Hypoxia regularly occurs during plant development and can be induced by the environment, for example through flooding. •To understand how plant tissue physiology responds to progressing oxygen restriction, we aimed at monitoring subcellular physiology in real-time and in vivo. We establish a fluorescent protein sensor-based system for multiparametric monitoring of dynamic changes in subcellular physiology of living Arabidopsis thaliana leaves, and exemplify its applicability for hypoxia stress. •By monitoring cytosolic dynamics of MgATP2-, free Ca2+ concentration, pH, NAD redox status and glutathione redox status in parallel, linked to transcriptional and metabolic responses, we generate an integrated picture of the physiological response to progressing hypoxia. We show that the physiological changes are surprisingly robust, even when plant carbon status is modified as achieved by sucrose feeding or extended night. Inhibition of the mitochondrial respiratory chain causes dynamics of cytosolic physiology that are remarkably similar to those under oxygen depletion, highlighting mitochondrial electron transport as key determinant of the cellular consequences of hypoxia beyond the organelle. •A broadly applicable system for parallel in vivo sensing of plant stress physiology is established to map out the physiological context under which both mitochondrial retrograde signaling and low oxygen signaling occur, indicating shared upstream stimuli. This article is protected by copyright. All rights reserved.