SoybeanAP1homologs control flowering time and plant height
SoybeanAP1homologs control flowering time and plant height
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大豆AP1同源物控制开花时间和株高
DOI：
10.1111/jipb.12988
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发表时间：
2020-07-24
影响因子：
11.4
通讯作者：
Dong, Lidong
中科院分区：
文献类型：
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作者：
Chen, Liyu;Nan, Haiyang;Dong, Lidong
开花时间和株高是直接影响大豆产量的重要农艺性状，APETALA 1（AP 1）是花器官发育的ABCE模型中的A类基因，有助于确定心皮、雄蕊、花瓣和萼片的身份。大豆中有4个AP 1同源基因，它们都主要在茎尖表达。在这里，我们使用成簇的规则间隔短回文重复序列（CRISPR）-CRISPR相关蛋白9技术来产生纯合的四重突变体gmap 1，在所有四个GmAP 1基因中具有功能缺失突变。在短日照（SD）条件下，gmap 1四倍体突变体开花延迟，花形态发生变化，节数和节间长度增加，植株比野生型高。相反，在SD条件下，与野生型相比，GmAP 1的过表达导致开花提前和植株高度降低。gmap 1突变体和过表达株系还表现出与开花和赤霉酸代谢相关的几个基因的表达改变，从而提供了对GmAP 1在控制大豆开花时间和株高的调控网络中的作用的深入了解。节数增加是最有希望提高大豆角果数和籽粒产量的性状。因此，这里描述的四个AP 1同源物的突变等位基因将是非常宝贵的分子育种提高大豆产量。
Flowering time and plant height are key agronomic traits that directly affect soybean (Glycine max) yield.APETALA1(AP1) functions as a class A gene in the ABCE model for floral organ development, helping to specify carpel, stamen, petal, and sepal identities. There are fourAP1homologs in soybean, all of which are mainly expressed in the shoot apex. Here, we used clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant,gmap1, with loss-of-function mutations in all fourGmAP1genes. Under short-day (SD) conditions, thegmap1quadruple mutant exhibited delayed flowering, changes in flower morphology, and increased node number and internode length, resulting in plants that were taller than the wild type. Conversely, overexpression ofGmAP1aresulted in early flowering and reduced plant height compared to the wild type under SD conditions. Thegmap1mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism, thereby providing insight into the role ofGmAP1in the regulatory networks controlling flowering time and plant height in soybean. Increased node number is the trait with the most promise for enhancing soybean pod number and grain yield. Therefore, the mutant alleles of the fourAP1homologs described here will be invaluable for molecular breeding of improved soybean yield.