Clonal dominance among T-lymphocyte infiltrates in arthritis.
Clonal dominance among T-lymphocyte infiltrates in arthritis.
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关节炎中 T 淋巴细胞浸润的克隆优势。
DOI：
10.1073/pnas.85.4.1179
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发表时间：
1988
影响因子：
11.1
通讯作者：
Kurnick,JT
中科院分区：
文献类型：
--
作者：
Stamenkovic,I;Stegagno,M;Wright,KA;Krane,SM;Amento,EP;Colvin,RB;Duquesnoy,RJ;Kurnick,JT
类风湿关节炎以及其他类型慢性破坏性炎症性关节炎患者的滑膜含有活化T淋巴细胞的浸润，这可能有助于疾病的发病机制。为了阐明这些浸润的性质，我们从14例晚期破坏性炎性关节疾病手术患者的滑膜碎片中培养出白细胞介素2 （IL-2）反应性T淋巴细胞。其中11个样本来自典型类风湿性关节炎患者，另外3个样本来自临床骨关节炎患者。对14个培养物中13个的t细胞受体(TCR) β链基因进行Southern blot分析，发现有明显的重排，表明每个培养物都以有限数量的克隆为优势。来自外周血的t细胞群受到多种激活剂的刺激，并通过IL-2扩增，在长期培养中没有显示出类似克隆的证据。这些结果表明，有限数量的活化t细胞克隆在类风湿滑膜组织损伤部位以及其他类型的破坏性炎性关节疾病中占主导地位。进一步表征这些t细胞克隆可能有助于我们了解这些风湿性疾病的发病机制。
Synovial membranes in patients with rheumatoid arthritis as well as other types of chronic destructive inflammatory arthritis contain infiltrates of activated T lymphocytes that probably contribute to the pathogenesis of the disease. In an effort to elucidate the nature of these infiltrates, interleukin 2 (IL-2)-responsive T lymphocytes were grown out of synovial fragments from 14 patients undergoing surgery for advanced destructive inflammatory joint disease. Eleven of the samples examined were from patients with classical rheumatoid arthritis, while three others were obtained from individuals with clinical osteoarthritis. Southern blot analysis of T-cell receptor (TCR) beta-chain genes in 13 of 14 cultures showed distinct rearrangements, indicating that each culture was characterized by the predominance of a limited number of clones. T-cell populations from peripheral blood stimulated with a variety of activators and expanded with IL-2 did not demonstrate evidence of similar clonality in long-term culture. These results suggest that a limited number of activated T-cell clones predominate at the site of tissue injury in rheumatoid synovial membranes as well as in other types of destructive inflammatory joint disease. Further characterization of these T-cell clones may aid our understanding of the pathogenesis of these rheumatic disorders.