lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett)
lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett)
复制标题
lnc94638是一个与葫芦条斑螟精子形成有关的睾丸特异性长链非编码RNA
DOI：
10.1111/imb.12729
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发表时间：
2021-08-04
影响因子：
2.6
通讯作者：
Wang, J-J
中科院分区：
文献类型：
--
作者：
Li, W-J;Wei, D.;Wang, J-J
长链非编码rna （Long non-coding rna, lncrna）通常表现为组织特异性分布，在许多物种中，睾丸特异性lncrna构成的lncrna比例最高。在这里，我们详细分析了瓜蝇（一种对葫芦科和其他相关作物具有高度破坏性的害虫）睾丸特异性lncrna。大多数睾丸特异性lncrna被发现是长基因间非编码rna （lincRNA）。这些lncrna的大小分布在600到1000个核苷酸之间。含有一个同工异构体编号和两个外显子的睾丸特异性lncrna数量最多。与其他男性组织相比，睾丸有更多高表达的lncrna。随机选取的10个睾丸特异性lncrna的实时定量聚合酶链反应结果显示，其表达模式与RNA-seq数据一致。进一步分析了高度表达的睾丸特异性lncRNA lnc94638。荧光原位杂交测定将lnc94638定位于含有成熟精子的睾丸顶端区域。RNA干扰介导的lnc94638基因表达下调可减少黄瓜雄性的精子数量，降低其生育能力。本研究提供了睾丸特异性lncrna的目录，表明睾丸特异性lnc94638参与精子发生，具有用于治疗男性不育的潜力。
Long non-coding RNAs (lncRNAs) generally display tissue-specific distributions, and testis-specific lncRNAs form the highest proportion of lncRNAs in many species. Here, we presented a detailed analysis of testis-specific lncRNAs in the melon fly, Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. Most testis-specific lncRNAs were found to be long intergenic non-coding RNAs (lincRNA). The size distribution of these lncRNAs ranged between 600 and 1000 nucleotides. Testis-specific lncRNAs that harboured one isoform number and two exons were the most abundant. Compared to other male tissues, the testis had more highly expressed lncRNAs. The quantitative real-time polymerase chain reaction results of 10 randomly selected testis-specific lncRNAs showed expression patterns consistent with RNA-seq data. Further analysis of the most highly expressed testis-specific lncRNA, lnc94638, was undertaken. Fluorescent in situ hybridization assays localized lnc94638 to the apical region of the testis that contains mature spermatozoa. RNA interference-mediated knockdown of lnc94638 expression reduced spermatozoa numbers and impaired the fertility of Z. cucurbitae male. This study provides a catalogue of testis-specific lncRNAs, shows that the testis-specific lnc94638 is involved in spermatogenesis and has the potential to be used for treating male sterility.