The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella
The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella
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DOI：
10.1371/journal.pone.0230767
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发表时间：
2020-07-30
期刊：
影响因子：
3.7
通讯作者：
Rowley, Paul A.
中科院分区：
文献类型：
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作者：
Fredericks, Lance R.;Lee, Mark D.;Rowley, Paul A.
用病原微生物注射实验室动物会造成重大的安全风险，因为可能会因意外针刺而受伤。这对于使用无脊椎动物疾病模型的研究人员来说尤其如此，因为注射需要精确度和准确度。大蜡螟幼虫（Galleria mellonella）的抑制通常是通过用手指和拇指紧紧抓住幼虫来实现的。防针手套或镊子可用于降低针刺风险，但可能导致动物受伤、通量损失和实验数据不一致。约束装置通常用于操纵小型哺乳动物，在这篇手稿中，我们描述了两种装置的构造，它们可用于在注射病原微生物之前捕获和约束大蜡螟。这些装置减少了幼虫的人工处理，并提供了一个工程控制，以防止意外针刺伤害，同时保持高注射率。
The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrainG.mellonellalarvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection.