PRODUCTION OF CITRIC AND OXALIC ACIDS AND SOLUBILIZATION OF CALCIUM-PHOSPHATE BY PENICILLIUM-BILAII
PRODUCTION OF CITRIC AND OXALIC ACIDS AND SOLUBILIZATION OF CALCIUM-PHOSPHATE BY PENICILLIUM-BILAII
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DOI：
10.1128/aem.58.5.1451-1458.1992
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发表时间：
1992-05-01
影响因子：
4.4
通讯作者：
KUIACK, C
中科院分区：
文献类型：
--
作者：
CUNNINGHAM, JE;KUIACK, C
研究了一株能增溶无机磷并促进植物吸收磷的拜莱青霉。使用含有磷酸钙和pH指示剂茜素红S的琼脂培养基，记录培养基组成对磷酸盐溶解度和培养基酸化的影响。在蔗糖硝酸盐液体培养基中，毕来氏拟青霉产生的主要酸性代谢产物为草酸和柠檬酸。柠檬酸的生产促进氮限制条件下，而草酸的生产促进碳限制条件下。柠檬酸在生长期和稳定期都有产生，但草酸只在稳定期产生。当通常产生酸的浸没培养物被诱导形成孢子时，培养基随着生长而向碱性而不是酸性反应转变。
An isolate of Penicillium bilaii previously reported to solubilize mineral phosphates and enhance plant uptake of phosphate was studied. Using agar media with calcium phosphate and the pH indicator alizarin red S, the influence of the medium composition on phosphate solubility and medium acidification was recorded. The major acidic metabolites produced by P. bilaii in a sucrose nitrate liquid medium were found to be oxalic acid and citric acid. Citric acid production was promoted under nitrogen-limited conditions, while oxalic acid production was promoted under carbon-limited conditions. Citric acid was produced in both growth and stationary phases, but oxalic acid production occurred only in stationary phase. When submerged cultures which normally produce acid were induced to sporulate, the culture medium shifted toward alkaline rather than acid reaction with growth.