Effect of object size and acoustic wavelength on pulsed ultrasound modulated fluorescence signals.

Effect of object size and acoustic wavelength on pulsed ultrasound modulated fluorescence signals.
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物体尺寸和声波波长对脉冲超声调制荧光信号的影响。

DOI:
10.1117/1.jbo.17.7.076008
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发表时间:
2012
影响因子:
3.5
通讯作者:
Huynh NT
Huynh NT
中科院分区:
医学3区
文献类型:
--
作者:
Huynh NT

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在混浊介质中检测超声调制的荧光是一个挑战,因为荧光强度低,非相干光的调制很弱。理论和实验研究的数量非常有限,据我们所知,这是脉冲US调制荧光层析成像的第一个演示。实验结果表明,检测信号依赖于声频和荧光目标沿超声传播轴线的大小。当物体沿声轴的长度是奇数个半波长时,检测信号的调制深度最大,而当物体是声波波长的整数倍时,检测信号的调制深度最弱。提供了嵌入22×13×30平方毫米散射凝胶模型(,)中的荧光管的图像,频率分别为1 MHz、1.5 MHz和2 MHz。根据对象的相对大小和频率,检测信号的调制深度变化5倍。该方法还通过在非散射凝胶中的一些简单实验和使用理论模型进行了验证。
Detection of ultrasound (US)-modulated fluorescence in turbid media is a challenge because of the low level of fluorescent light and the weak modulation of incoherent light. A very limited number of theoretical and experimental investigations have been performed, and this is, to our knowledge, the first demonstration of pulsed US-modulated fluorescence tomography. Experimental results show that the detected signal depends on the acoustic frequency and the fluorescent target’s size along the ultrasonic propagation axis. The modulation depth of the detected signal is greatest when the length of the object along the acoustic axis is an odd number of half wavelengths and is weakest when the object is an integer multiple of an acoustic wavelength. Images of a fluorescent tube embedded within a 22- by 13- by 30 mm scattering gel phantom (,) with 1-, 1.5-, and 2 MHz frequency US are presented. The modulation depth of the detected signal changes by a factor of 5 depending on the relative size of the object and the frequency. The approach is also verified by some simple experiments in a nonscattering gel and using a theoretical model.
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