Primary amines and chloroquine inhibit cytotoxic responses to Shigella toxin and permit late antibody rescue of toxin treated cells.
Primary amines and chloroquine inhibit cytotoxic responses to Shigella toxin and permit late antibody rescue of toxin treated cells.
复制标题
伯胺和氯喹抑制志贺氏菌毒素的细胞毒性反应，并允许后期抗体拯救毒素处理的细胞。
DOI：
10.1016/0006-291x(84)90689-2
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发表时间：
1984
影响因子：
3.1
通讯作者：
Jacewicz,M
中科院分区：
文献类型：
--
作者：
Keusch,GT;Jacewicz,M
通过测定细胞毒性和晚期抗体拯救来确定溶酶体促剂和转氨酶的伯胺抑制剂对HeLa细胞中志贺氏菌毒素活性的影响。测试的所有试剂均导致显著的抗体拯救，但在不存在添加的抗体的情况下，仅氯化铵、丹酰尸胺、腐胺、杆菌肽、丝氨酸/硼酸盐缓冲液和氯喹具有抑制作用。这些化合物似乎在内吞液泡内起作用和/或抑制毒素从细胞表面转移到胞质溶胶。这些数据与ST通过受体介导的内吞作用从细胞表面易位到胞质溶胶的机制一致。
The effect of lysosomotropic agents and primary amine inhibitors of transglutaminase on Shigella toxin activity in HeLa cells was determined by measuring cytotoxicity and late antibody rescue. All agents tested resulted in significant antibody rescue, but only ammonium chloride, dansylcadaverine, putrescine, bacitracin, serine/borate buffer, and chloroquine were inhibitory in the absence of added antibody. These compounds appear to be acting within the endocytic vacuole and/or inhibiting translocation of toxin from the cell surface to the cytosol. These data are consistent with a mechanism of translocation of ST from the cell surface to the cytosol by the process of receptor mediated endocytosis.