Sustainabitity of mechanically lengthened bowel in rats
Sustainabitity of mechanically lengthened bowel in rats
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DOI：
10.1016/j.jpedsurg.2006.08.014
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发表时间：
2006-12-01
影响因子：
2.4
通讯作者：
Dunn, James C. Y.
中科院分区：
文献类型：
--
作者：
Chang, Paut C. Y.;Mendoza, Julianne;Dunn, James C. Y.
导读：有研究表明，通过逐渐的机械拉伸，肠段的长度可以增加一倍。本研究评估拉长的肠段在移除拉伸装置后是否保留结构和功能。方法：选取20只大鼠，分离出1.5 cm的空肠段。将螺钉插入离体空肠段5mm，每周3次，直至螺钉拉伸3cm，取出螺钉。三周后，取出空肠段进行分析。对加长的空肠节段进行了比较。结果：空肠段在逐渐拉伸后长度增加一倍，并在螺钉取出后3周内保持该长度（3.1 +/- 0.8 vs 3.2 +/- 0.4 cm, P < 0.05）。取下螺钉后3周，拉伸空肠段绒毛高度、肌肉厚度、总碱性磷酸酶和乳糖酶活性均无变化。结论：机械力诱导大鼠离体空肠节段持续延长。组织学和酶的改变在去除机械力后也持续了3周。这一现象可能为短肠综合征的治疗提供一种新的方法。(c) 2006爱思唯尔公司版权所有。
Introduction: It has been shown that the length of an intestinal segment may be doubled by applying gradual mechanical stretching. This study evaluated whether the lengthened intestinal segment retained the structure and function after the stretching device was removed.Methods: A 1.5-cm jejunal segment was separated from intestinal continuity in 20 rats. After advancing a screw into the isolated jejunal segment by 5 mm 3 times a week until it was stretched by 3 cm, the screw was removed. Three weeks later, the jejunal segments were retrieved for analyses. Comparisons were made between the lengthened jejunal segments.Results: The jejunal segment doubled its length after gradual stretching and retained this length 3 weeks after the screw removal (3.1 +/- 0.8 vs 3.2 +/- 0.4 cm, P >.05). The villous height, the muscular thickness, and the total alkaline phosphatase and lactase activities of the stretched jejunal segments were also unchanged 3 weeks after the screw removal.Conclusions: Mechanical force induced the sustained lengthening of isolated jejunal segments in rats. The histologic and enzymatic alterations also persisted 3 weeks after the mechanical force was removed. This phenomenon may provide a novel method for the treatment of short bowel syndrome. (c) 2006 Elsevier Inc. All rights reserved.