Calcium ion transport in higher plant mitochondria (Helianthus tuberosus)
Calcium ion transport in higher plant mitochondria (Helianthus tuberosus)
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高等植物线粒体（Helianthus tuberosus）中的钙离子转运
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
D. Zannoni
中科院分区：
文献类型：
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作者：
M. Rugolo;R. Pistocchi;D. Zannoni
耶路撒冷线粒体Ca ~（2+）转运的研究CV. OB 1）块茎。通过跟踪呼吸条件下Ca 2+的分布，我们已经能够表明Ca 2+在基质空间中的积累取决于膜电位（Δ P），因为这种摄取不受质子载体尼日利亚菌素的影响，但完全被缬氨霉素和羰基氰-对三氟甲氧基苯腙（FCCP）阻断。Ca 2+吸收需要磷酸盐（Pi），并被汞撒利和钌红（RR）抑制。除Ca ~（2+）内流途径外，H. tuberosus具有RR不敏感的Ca ~（2+）外排途径，不受外界Na ~+的刺激，在离子载体如A23187和缬氨霉素以及解偶联剂FCCP存在下，Ca ~（2+）从负载Ca ~（2+）的线粒体中迅速释放。Pi-转运抑制剂mersalyl也通过逆转摄取途径诱导大量Ca 2+释放，后一过程被RR阻断。因此，耶路撒冷线粒体具有不同于动物线粒体和某些其它植物物种的Ca 2+循环机制。
A study on Ca2+ transport by mitochondria isolated from Jerusalem artichoke (Helianthus tuberosus L. cv. OB1) tubers is presented. By following the distribution of Ca2+ under respiratory conditions, we have been able to show that Ca2+ accumulation into the matrix space depends on membrane potential (ΔΨ) since the uptake is not affected by the protonophore nigericin but fully blocked by valinomycin and carbonyl cyanide-p-trifluoromethoxy phenylhydrazone (FCCP). Ca2+ uptake requires phosphate (Pi) and is inhibited by mersalyl and by ruthenium red (RR). In addition to a Ca2+ influx route, mitochondria from H. tuberosus possess an RR-insensitive Ca2+ efflux pathway which is not stimulated by external Na+, Ca2+ is rapidly released from Ca2+-loaded mitochondria in the presence of ionophores such as A23187 and valinomycin and of the uncoupler FCCP. The Pi-transport inhibitor mersalyl also induces a massive Ca2+ release through reversal of the uptake route, the latter process being blocked by RR. Thus Jerusalem artichoke mitochondria possess a Ca2+ cycling mechanism which is different from that of animal mitochondria and certain other plant species.
地尔硫卓和某些其他 Ca2 拮抗剂药物选择性抑制 Na 诱导的心脏线粒体 Ca2 释放。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Vághy,PL;Johnson,JD;Matlib,MA;Wang,T;Schwartz,A
通讯作者：
Schwartz,A
DOI：
10.1021/je060146z
发表时间：
2007-01-01
期刊：
JOURNAL OF CHEMICAL AND ENGINEERING DATA
影响因子：
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作者：
Costa, Mariana C.;Rolemberg, Marlus P.;Meirelles, Antonio J. A.
通讯作者：
Meirelles, Antonio J. A.