Biosynthesis and Signal Transduction of ABA, JA, and BRs in Response to Drought Stress of Kentucky Bluegrass
Biosynthesis and Signal Transduction of ABA, JA, and BRs in Response to Drought Stress of Kentucky Bluegrass
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ABA、JA 和 BR 响应肯塔基早熟禾干旱胁迫的生物合成和信号转导
DOI：
10.3390/ijms20061289
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发表时间：
2019-03-21
影响因子：
5.6
通讯作者：
Zhang, Lu
中科院分区：
文献类型：
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作者：
Chen, Yajun;Chen, Yang;Zhang, Lu
肯塔基州早熟禾（Poapratensis）是冷季型草坪草中应用最广泛的一种，但对干旱胁迫较为敏感。KB的分子研究受到其庞大而复杂的基因组结构的阻碍。在这项研究中，进行了短期和长期缺水之间的比较转录组学研究。构建了三个转录组文库，然后使用PEG 6000处理后0、2和16 h的植物叶片RNA样品进行测序。共发现199，083个差异表达基因（DEG），京都基因与基因组百科全书（KEGG）注释显示，DEG主要集中在“植物激素信号转导”和“MAPK信号通路-植物”两个方面。其中PYL、JAZ和BSK等基因参与了脱落酸、茉莉酸和油菜素内酯等激素的信号转导过程，可能在KB应对干旱胁迫中发挥重要作用。随着干旱时间的延长，阿坝、JA和BR含量显著增加。这些特异性DEG编码的功能蛋白、激酶和转录因子，为进一步开展KB耐旱性的遗传操作提供了有价值的信息。
Kentucky bluegrass (KB, Poa pratensis) is one of the most widely used cool-season turfgrass species, but it is sensitive to drought stress. Molecular studies in KB are hindered by its large and complex genome structure. In this study, a comparative transcriptomic study was conducted between a short and long period of water deficiency. Three transcriptome libraries were constructed and then sequenced by using leaf RNA samples of plants at 0, 2, and 16 h after PEG6000 treatment. A total of 199,083 differentially expressed genes (DEGs) were found. The Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed that DEGs were enriched in “Plant hormone signal transduction” and “MAPK signaling pathway-Plant”. Some key up-regulated genes, including PYL, JAZ, and BSK, were involved in hormone signaling transduction of abscisic acid, jasmonic acid, and brassinosteroid and possibly these genes play important roles in coping with drought stress in KB. Furthermore, our results showed that the concentrations of ABA, JA and BR increased significantly with the extension of the drought period. The specific DEGs encoding functional proteins, kinase and transcription factors, could be valuable information for genetic manipulation to promote drought tolerance of KB in the future.