Quantitation and typing of serum hepatitis C virus RNA in patients with chronic hepatitis C treated with interferon‐β
Quantitation and typing of serum hepatitis C virus RNA in patients with chronic hepatitis C treated with interferon‐β
复制标题
干扰素-β治疗慢性丙型肝炎患者血清丙型肝炎病毒RNA的定量和分型
DOI：
10.1002/hep.1840180606
复制
发表时间：
1993
期刊：
影响因子：
13.5
通讯作者：
Shiro Suzuki
中科院分区：
文献类型：
--
作者：
Yoshinao Kobayashi;Shozo Watanabe;M. Konishi;M. Yokoi;R. Kakehashi;M. Kaito;Masahiro Kondo;Yuji Hayashi;T. Jomori;Shiro Suzuki
我们对接受β-干扰素治疗的慢性丙型肝炎患者进行了血清丙型肝炎病毒RNA滴度定量和丙型肝炎病毒亚型测定，以研究血清ALT应答、血清丙型肝炎病毒滴度和丙型肝炎病毒亚型之间的关系。在146例接受干扰素-β治疗的慢性丙型肝炎患者中，随机选择24例持续血清ALT正常化的患者（完全应答者）和26例血清ALT未正常化的患者（无应答者）。通过“单管”聚合酶链反应方法进行丙型肝炎病毒的检测、分型和定量。在24例完全应答者中，21例（87.5%）变为丙型肝炎病毒RNA阴性，而26例无应答者中有21例（80.8%）保持阳性。丙型肝炎病毒I、II、III、IV、II + III和III + IV型感染分别为0（0%）、22（51.2%）、10（23.3%）、1（2.3%）、7（16.5%）和3（7.9%）例。完全应答者治疗前HCV RNA滴度（0.4 ± 2.0 × 104 CID 50/ml）显著低于无应答者（3.8 ± 4.5 × 104 CID 50/ml）（P < 0.01）。无论HCV亚型如何，HCV感染超过104 CID 50/ml的患者血清ALT均未恢复正常，而HCV感染低于102 CID 50/ml的大多数患者血清ALT完全恢复。这些结果表明，不同丙型肝炎病毒亚型的混合感染似乎比以前报道的更常见，治疗前血清丙型肝炎病毒RNA水平是一个更重要的预测干扰素治疗的结果比病毒基因型。（《肝脏学》1993年;18：1319-1325）
We quantified serum hepatitis C virus RNA titers and determined hepatitis C virus subtypes in chronic hepatitis C patients treated with interferon‐β to investigate relationships among serum ALT response, serum hepatitis C virus titer and hepatitis C virus subtype. Of 146 chronic hepatitis C patients who received interferon‐β therapy, 24 patients with sustained serum ALT normalization (complete responders) and 26 patients without serum ALT normalization (nonresponders) were randomly selected. Detection, typing and quantitation of hepatitis C virus were performed by means of the “single‐tube” polymerase chain reaction method. Of the 24 complete responders, 21 (87.5%) became negative for hepatitis C virus RNA, whereas 21 (80.8%) of the 26 nonresponders remained positive. Hepatitis C virus infections with types I, II, III, IV, II + III and III + IV occurred in 0 (0%), 22 (51.2%), 10 (23.3%), 1 (2.3%), 7 (16.5%) and 3 (7.9%) patients, respectively. The mean pretreatment hepatitis C virus RNA titer of complete responders (0.4 ± 2.0 × 104 CID50/ml) was significantly lower than that of nonresponders (3.8 ± 4.5 × 104 CID50/ml) (p < 0.01). Regardless of HCV subtype, patients with more than 104 CID50/ml of HCV did not show serum ALT normalization, whereas complete serum ALT response was seen in most cases with less than 102 CID50/ml HCV. These results show that mixed infections with different hepatitis C virus subtypes appear to be more common than previously reported and that the pretreatment serum level of hepatitis C virus RNA is a more important predictor of outcome of interferon therapy than is virus genotype. (HEPATOLOGY 1993;18:1319–1325.)