The cell cycle dependence of protein synthesis during Xenopus laevis development.
The cell cycle dependence of protein synthesis during Xenopus laevis development.
复制标题
非洲爪蟾发育过程中蛋白质合成的细胞周期依赖性。
DOI：
10.1016/0012-1606(91)90460-k
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
Newport,JW
中科院分区：
文献类型：
--
作者：
Kanki,JP;Newport,JW
The regulation of early embryonic development in the amphibianXenopus laevisdepends largely upon translational and post-translational regulatory mechanisms to direct the complex cytodifferentiations that take place during early cleaage and blastula formation. The cell cycle dependence of protein synthesis was examined in developingXenopusembryos as well as in cycling cell-free lysates fromXenopuseggs. In both cases M-phase and the activation of the M-phase kinase were found to be correlated with an inhibition of translation. Translation in both the rough endoplasmic reticulum and cytosolic-free ribosomes were affected by this inhibition. Since elongation was found to be unaffected by M-phase, shifts in the polysome profiles during M-phase indicated that the inhibition affected initiation processes. The activity of the M-phase kinase may inhibit initiation through the modification of initiation factors or some other component during this process. The cell cycle dependence of translation may affect developmental mechanisms controlled by the titration of regulatory proteins.
