HIGH-PERFORMANCE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL CAPILLARY ELECTROPHORESIS OF PEPTIDES AND PROTEINS
HIGH-PERFORMANCE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL CAPILLARY ELECTROPHORESIS OF PEPTIDES AND PROTEINS
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DOI：
10.1016/s0021-9673(01)85026-3
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发表时间：
1987-06-26
期刊：
影响因子：
--
通讯作者：
KARGER, BL
中科院分区：
文献类型：
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作者：
COHEN, AS;KARGER, BL
高效毛细管十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）已发展用于肽和蛋白质的分离和分子量（MW）测定。本文报道了丙烯酰胺在内径为75 μm的熔融石英毛细管中的聚合反应。10或20厘米长。将毛细管置于检测器的光路中进行在线紫外检测。肌红蛋白片段和标准蛋白质的混合物的快速分离被证明与效率的顺序为40 000板的柱，20厘米长。观察到SDS蛋白复合物的log MW与迁移率的线性图。聚丙烯酰胺单体组成百分比（% T）变化产生预期对数迁移率与%的共同截距T，证实了凝胶毛细管中的尺寸分离机制。用10 cm柱在10 min内分离胰岛素的A和B链。
High-performance capillary sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed for the separation and molecular weight (MW) determination of peptides and proteins. In this work, acrylamide was polymeized in fused-silica capillaries of 75 μm I.D. and 10 or 20 cm length. On-line UV detection was employed by placing the capillary in the optical path of the detector. Rapid separations of myoglobin fragments and of a mixture of standard proteins were demonstrated with efficiencies of the order of 40 000 plates for a column, 20 cm in length. Linear plots of log MWvs.mobility for the SDS protein complexes were observed. Variation in the percentage monomer compositin (% T) of the polyacrylamide yielded the expected common intercept of log mobilityvs.% T, confirming the size separation mechanism in the gel capillaries. The separation of the A and B chains of insulin with a 10-cm column in less than 10 min was also demonstrated.