Detecting Lineage-Specific Marker Genes for Tumor Evolution Based on Single Cell Transcriptome
Detecting Lineage-Specific Marker Genes for Tumor Evolution Based on Single Cell Transcriptome
复制标题
DOI：
10.17706/ijbbb.2021.11.3.50-57
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kosho Murayama;H. Matsuda
中科院分区：
文献类型：
--
作者：
Kosho Murayama;H. Matsuda
标记基因检测对于理解肿瘤进展是重要的，因此，本文提出了一种新的方法来检测肿瘤细胞进展中几个谱系中每一个的标记基因。该方法将肿瘤细胞聚类成反映药物敏感性、耐药性和其他特征的组，并推断谱系随着肿瘤进展和潜在分支沿着经历过渡过程。该方法估计每个谱系进展过程中的连续基因表达，并通过分析谱系内和谱系间的差异表达来识别标记基因。利用这种方法，我们成功地检测到一个潜在的标记基因与肿瘤耐药，这是没有检测到的传统的基于聚类的方法。
Marker gene detection is important for understanding tumor progression; therefore, this paper proposes a novel approach to detect marker genes for each of several lineages as tumor cells progress. The approach clusters the tumor cells into groups that reflect the drug sensitivity, resistance, and other characteristics and infers lineages undergoing transitional processes along with tumor progression and potential branching. The approach estimates continuous gene expression during the progression of each lineage and identifies marker genes by analyzing within-lineage and between-lineage differential expression. Using this method, we successfully detected a potential marker gene related to tumor drug resistance, which was not detected by a conventional cluster-based method.