Time-correlated single-photon counting with alternate recording of excitation and emission
Time-correlated single-photon counting with alternate recording of excitation and emission
复制标题
时间相关的单光子计数以及交替记录激发和发射
DOI：
10.1016/0022-2313(89)90051-3
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发表时间：
1989
影响因子：
3.6
通讯作者：
A. Läufer
中科院分区：
文献类型：
--
作者：
H. Dreeskamp;T. Salthammer;A. Läufer
为了最大限度地减少闪光灯不稳定性对用时间相关单光子计数技术测定荧光衰减曲线的影响，建议交替记录激发和发射曲线。在这里描述的设备中，这是通过安装在水平板上的两个石英棱镜改变光路来实现的，并移动到交替的位置，而样品保持固定。与已报道的其他方法相比，这种设计非常简单，因为样品保持器保持不变。使用分别具有几乎与温度无关和强烈与温度相关的寿命的含菲和二中位取代菲的流体溶液，通过分析双指数衰减曲线来测试该装置的效率。
In order to minimize the effect of flashlamp instabilities in determining fluorescence decay profiles by the time-correlated single-photon counting technique, an alternate recording of excitation and emission profiles is advisable. In the equipment described here, this is performed by changing the light path via two quartz prisms mounted on a horizontal plate and moved into alternate positions, while the sample remains fixed. This design is extremely simple compared to other methods reported, since the sample holder is left unchanged.The efficiency of the equipment was tested by the analysis of double exponential decay curves using fluid solutions containing anthracene and di-meso-substituted anthracenes, which have almost temperature-independent and strongly temperature-dependent lifetimes respectively.