Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases
Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases
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DOI：
10.1016/s0378-8741(02)00322-7
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发表时间：
2003-02-01
影响因子：
5.4
通讯作者：
Mukherjee, B
中科院分区：
文献类型：
--
作者：
Auddy, B;Ferreira, M;Mukherjee, B
许多印度药用植物已经在传统医学系统（阿育吠陀）中使用了数千年。其中包括阿育吠陀从业者用于治疗神经退行性疾病如帕金森氏症、阿尔茨海默氏症、记忆丧失、神经变性和其他神经元疾病的植物。虽然神经变性疾病的病因学仍然是谜，但有证据表明，有缺陷的能量代谢、兴奋性毒性和氧化损伤可能是关键因素（Ann.Neurol. 38（3）（1995）357）。阿育吠陀系统的一部分，提供了一种预防和治疗退行性疾病的方法，被称为Rasayana，用于此目的的植物被归类为返老还童。这类植物通常具有很强的抗氧化活性（Pharmacol.生物化学行为43（1992）1175），但只有少数已被详细研究。在本研究中，三个这样的rasayana植物的毒性和自由基清除活性在体外和离体的第一次进行了测试。所有三种植物输注液（高达1 mg/ml）对PC 12细胞系的存活率均无毒性作用，如MTT试验所判断。用2，2 '-连氮双-3-乙基-苯并噻唑啉-6-磺酸（ABTS（.+））对该植物的乙醇提取物和水浸提物进行抗氧化活性测试。自由基阳离子脱色测定;使用大鼠脑匀浆的自发脂质过氧化进行植物输液对脂质过氧化的抑制，并测定IC 50值。ABTS试验的结果显示，黄花稔的乙醇提取物被发现是最有效的（IC 50 16.07 μ g/ml），其次是Evolvulus alsinoides（IC 50 33.39 μ g/ml）和狗牙根（IC 50 78.62 μ g/ml）。水浸液的相对抗氧化能力按以下顺序观察：E。Alsinoides（IC_（50）172.25 μ g/ml）> C dactylon（IC_（50）273.64 μ g/ml）> S. Cordifolia（IC_（50）342.82 μ g/ml）。水处理对膜脂过氧化作用的影响为：川滇紫草（IC_（50）89.23 μ g/ml）>川滇紫草（IC_（50）89.23 μ g/ml）; Cordifolia）（IC 50 126.78 μ g/ml）> C dactylon（IC 50 608.31 μ g/ml）。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
A number of Indian medicinal plants have been used for thousands of years in the traditional system of medicine (Ayurveda). Amongst these are plants used for the management of neurodegenerative diseases such as Parkinson's, Alzheimer's, loss of memory, degeneration of nerves and other neuronal disorders by the Ayurvedic practitioners. Though the etiology of neurodegenerative diseases remains enigmatic, there is evidence, which indicates that defective energy metabolism, excitotoxicity and oxidative damage may be crucial factors (Ann. Neurol. 38 (3) (1995) 357). The part of the Ayurvedic system that provides an approach to prevention and treatment of degenerative diseases is known as Rasayana, and plants used for this purpose are classed as rejuvenators. This group of plants generally possesses strong antioxidant activity (Pharmacol. Biochem. Behav. 43 (1992) 1175), but only a few have been investigated in detail. In the present study, three such rasayana plants were tested for the first time for their toxicity and free radical scavenging activity both in vitro and ex vivo. All the three plant infusions (up to 1 mg/ml) showed no toxic effects on the viability of PC12 cell line as judged by MTT-test. Both ethanolic extracts and water infusions of the plants were tested for their antioxidant activity in the 2,2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS(.+)) radical cation decolorization assay; inhibition of lipid peroxidation by plant infusions was carried out using spontaneous lipid peroxidation of rat brain homogenate, and IC50 values were determined. The results from the ABTS assay showed that the ethanolic extract of Sida cordifolia was found to be most potent (IC50 16.07 mug/ml), followed by Evolvulus alsinoides (IC50 33.39 mug/ml) and Cynodon dactylon (IC50 78.62 mug/ml). The relative antioxidant capacity for the water infusions was observed in the following order: E. alsinoides (IC50 172.25 mug/ml) > C dactylon (IC50 273.64 mug/ml) > S. cordifolia (IC50 342.82 mug/ml). The results of water infusions of the plants on lipid peroxidation were as follows: E alsinoides (IC50 89.23 mug/ml) > S. cordifolia) (IC50 126.78 mug/ml) > C dactylon (IC50 608.31 mug/ml). (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.