Molecular insights into the functional role of myoglobin.
Molecular insights into the functional role of myoglobin.
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DOI：
10.1007/978-0-387-75434-5_14
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发表时间：
2007
影响因子：
--
通讯作者：
D. Garry;P. Mammen
中科院分区：
文献类型：
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作者：
D. Garry;P. Mammen
肌红蛋白是一种细胞质血红蛋白，仅限于心肌细胞和氧化骨骼肌纤维。肌红蛋白是一种特性良好的蛋白质，大量研究已证实它在促进横纹肌中的氧运输方面具有重要作用。最近使用基因破坏技术的策略已经产生了缺乏肌红蛋白的小鼠。这些肌红蛋白缺陷小鼠具有二元表型，并且这些突变小鼠的亚群是可存活且可生育的。对可存活肌红蛋白缺失小鼠的表征揭示了许多分子和细胞适应性机制，这些机制可促进突变横纹肌细胞中的氧输送。此外，使用肌红蛋白缺陷小鼠模型进行的细胞和生理学研究支持这样的结论：肌红蛋白的功能包括：促进氧运输、储存氧气以及一氧化氮或活性氧的清除剂。总的来说，遗传小鼠模型的使用将进一步增强我们对正常和病理肌肉谱系中肌红蛋白功能的理解。
Myoglobin is a cytoplasmic hemoprotein that is restricted to cardiomyocytes and oxidative skeletal muscle fibers. Myoglobin is a well-characterized protein and numerous studies have established that it has an essential role in facilitated oxygen transport in striated muscles. Recent strategies, using gene disruption technologies, have produced mice that lack myoglobin. These myoglobin deficient mice have a binary phenotype and a subpopulation of these mutant mice is viable and fertile. Characterization of the viable myoglobin null mice has uncovered a number of molecular and cellular adaptive mechanisms that function to promote oxygen delivery in the mutant striated muscle cell. Moreover, cellular and physiological studies, using the myoglobin deficient mouse model, support the conclusion that the functions of myoglobin include: facilitated oxygen transport, the storage of oxygen and a scavenger of nitric oxide or reactive oxygen species. Collectively, the use of genetic mouse models will further enhance our understanding of myoglobin function in normal and pathological muscle lineages.