Purification and serology of bean yellow mosaic virus.
Purification and serology of bean yellow mosaic virus.
复制标题
豆黄花叶病毒的纯化及血清学分析。
DOI：
10.3186/jjphytopath.41.192
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
D. Murayama
中科院分区：
文献类型：
--
作者：
I. Uyeda;M. Kojima;D. Murayama
蚕豆黄花叶病毒（BYMV）30号分离物经0.1M Tris-HCl缓冲液（pH7.0）提取、四氯化碳澄清、4%聚乙二醇沉淀、差速离心和蔗糖密度梯度离心，得到纯化的病毒。ISCO分馏器扫描模式表明纯度很高，但血清学结果显示存在一些宿主污染。病毒产量约为2 mg/100 g蚕豆叶片，抗30号分离物血清的环状沉淀效价为1/2048。虽然完整的病毒颗粒不能在琼脂凝胶平板中扩散，但当向琼脂或病毒悬液中加入0.5%SDS（或LIS）时，观察到清晰的沉淀线。在琼脂扩散试验中，将30号分离物与BYMV的坏死株系和褪绿斑点株系进行了血清学比较。分离物30与这些菌株之间观察到了刺突反应。
Isolate No.30 of bean yellow mosaic virus (BYMV) was purified from broad bean leaves by extraction with 0.1M Tris-HCl buffer, pH7.0, containing 0.05M EDTA and 1% 2-mercaptoethanol; repeated clarification with carbon tetrachloride and precipitation with 4% polyethylene glycol (#6, 000) followed by differential centrifugation and sucrose density-gradient centrifugation. ISCO fractionator scanning patterns indicated a high degree of purity but serological results revealed the presence of some host contaminates. The yield of virus was about 2mg/100g of broad bean leaves.The titer of the antiserum against isolate No.30 was 1/2048 by ring precipitin tests. Although the intact virus particles could not diffuse in agar gel plates, a clear precipitin line was observed when 0.5% SDS (or LIS) was added to the agar or virus suspension. Isolate No.30 was compared serologically with a necrotic strain and a chlorotic spot strain of BYMV in agar gel diffusion tests. Spur reactions were observed between isolate No.30 and these strains.